Penetration of peripheral glucose and insulin into cerebrospinal fluid in rats.
Penetration of peripheral glucose and insulin into cerebrospinal fluid in rats.
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DOI：
10.1152/ajpregu.1988.255.2.r200
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发表时间：
1988-08
期刊：
影响因子：
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通讯作者：
A. Steffens;A. Scheurink;D. Porte;S. Woods
中科院分区：
文献类型：
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作者：
A. Steffens;A. Scheurink;D. Porte;S. Woods
在这项研究中，血浆胰岛素和葡萄糖渗透到脑脊液（CSF）进行了研究。在大鼠枕大池和左右颈静脉中植入套管。自由活动的大鼠在4小时内静脉输注葡萄糖溶液（10 mg/min）或生理盐水。在输注之前、期间和之后，同时采集血液和CSF样本。输注葡萄糖导致血浆葡萄糖和胰岛素立即升高。虽然CSF葡萄糖后，血浆葡萄糖在10分钟内，CSF胰岛素不变，直到40分钟。葡萄糖输注终止后，所有物质的水平在10分钟内恢复到基线。24小时的食物剥夺导致血糖，血浆胰岛素，CSF葡萄糖，CSF胰岛素显着下降。在剥夺后开始进食时，血浆葡萄糖和胰岛素在10分钟内增加，而CSF葡萄糖延迟10至40分钟，之后达到或超过随意值。CSF胰岛素始终保持在自由水平以下。结论：1）葡萄糖和胰岛素渗透到CSF中，2）CSF胰岛素和葡萄糖可以实现假定的反馈，在食物摄入和体重的稳态控制中。
In this study the penetration of plasma insulin and glucose into the cerebrospinal fluid (CSF) was investigated. Rats were implanted with cannulas in the cisterna magna and into the left and right jugular veins. Freely moving rats were intravenously infused during 4 h with either glucose solution (10 mg/min) or saline. Before, during, and after the infusions, simultaneous blood and CSF samples were taken. Infusion of glucose led to an immediate rise of both plasma glucose and insulin. Although CSF glucose followed plasma glucose within 10 min, CSF insulin was unchanged until 40 min. After termination of the glucose infusion, levels of all substances returned to base line within 10 min. Twenty-four-hour food deprivation resulted in a significant decrease of plasma glucose, plasma insulin, CSF glucose, and CSF insulin. At the onset of eating after deprivation, an increase of plasma glucose and insulin occurred within 10 min, whereas CSF glucose was delayed between 10 and 40 min, after which ad libitum values were attained or surpassed. CSF insulin always remained below ad libitum levels. It is concluded that 1) glucose and insulin penetrate into the CSF and 2) CSF insulin and glucose can fulfill a putative feedback in homeostatic control of food intake and body weight.