Neuronal localization of a novel mosaic apolipoprotein E receptor, LR11, in rat and human brain
Neuronal localization of a novel mosaic apolipoprotein E receptor, LR11, in rat and human brain
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DOI：
10.1016/s0006-8993(99)01542-5
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发表时间：
1999-07-03
期刊：
影响因子：
2.9
通讯作者：
Ikeda, K
中科院分区：
文献类型：
--
作者：
Motoi, Y;Aizawa, T;Ikeda, K
最近发现的一种新型马赛克蛋白是低密度脂蛋白受体(LDLR)家族的新成员，命名为LR11。LR11转录本在脑组织中的主要表达和神经黏附分子中发现的元件的存在提示了中枢神经系统的一种功能(S)。为了深入了解中枢神经系统中的这种复杂受体，我们提出了一种兔多克隆抗体，并对大鼠和人脑组织进行了免疫组织化学检测。在这两个物种中，LR11免疫反应主要分布在整个大脑的神经元中。大鼠大脑的详细图谱显示，LR11免疫反应活性在广泛的神经元群体中分布，尽管不同位置的强度有所不同。免疫反应最强的是海马神经元、部分脑干核团和浦肯野细胞，丘脑和下丘脑神经元染色较弱。独一无二的是，LR11免疫反应阳性细胞质点状分布于顶端树突附近，以海马锥体神经元最为明显。在人脑中，在单个神经元内可以看到一到四个免疫反应点。LR11的神经元定位及其与胞浆结构的独特联系，可能暗示了LR11在中枢神经某些神经元群体的脂蛋白代谢和细胞内转运中的作用。(C)1999 Elsevier Science B.V.保留所有权利。
A new type of mosaic protein was recently discovered as a new member of the low density lipoprotein receptor (LDLR) family, designated as LR11. The predominant expression of LR11 transcripts in brain tissue and the presence of elements found in neural adhesion molecules suggested a function(s) in the central nervous system (CNS). In order to gain insight about this complex receptor in the CNS, we raised a rabbit polyclonal antibody and examined immunohistochemically rat and human brain tissue. A strong LR11 immunoreactivity was found to be localized mainly in neurons throughout the brain in both species. A detailed mapping in the rat brain showed a distribution of LR11 immunoreactivity in a widespread population of neurons, though the intensity varied between different locations. The most prominent immunoreactivity was observed in neurons of the hippocampus, some nuclei of brain stem and Purkinje cells, whereas neurons of the thalamus and the hypothalamus showed weak staining. Uniquely, the single LR11 immunoreactive cytoplasmic puncta were observed in the proximity of apical dendrites, most conspicuously in the pyramidal neurons of hippocampus. In the human brain, one to four immunoreactive puncta were seen within individual neurons. The neuronal localization of LR11 and its unique association of cytoplasmic structure, presumably botrysome, may suggest the roles of LR11 in both the lipoprotein metabolism and intracellular trafficking in certain neuronal population of the CNS. (C) 1999 Elsevier Science B.V. All rights reserved.