Cell adhesion and tissue response to hydroxyapatite nanocrystal-coated poly(L-lactic acid) fabric
Cell adhesion and tissue response to hydroxyapatite nanocrystal-coated poly(L-lactic acid) fabric
复制标题
DOI：
10.1016/j.jbiosc.2009.04.003
复制
发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Takakuda, Kazuo
中科院分区：
文献类型：
--
作者：
Yanagida, Hiroshi;Okada, Masahiro;Takakuda, Kazuo
研究了纳米羟基磷灰石（HAp）涂层聚L-乳酸（PLLA）织物对细胞粘附和组织反应的影响。HAp纳米晶体通过湿化学方法制备，然后在800 ℃下用抗烧结剂煅烧以防止煅烧诱导的烧结。PLLA织物用碱性水溶液水解后，HAp纳米晶体通过HAp上的钙离子与碱处理的PLLA上的羧基之间的离子相互作用而被包覆。通过扫描电镜观察到HAp纳米晶均匀地包覆在PLLA表面。通过FT-IR估计HAp和PLLA之间的离子相互作用。通过使用小鼠成纤维细胞系L929的体外测试，证明了HAp纳米颗粒涂覆的表面的细胞粘附性的改善。此外，通过大鼠皮下植入试验证实了对HAp纳米纤维涂覆的PLLA织物的炎症反应降低（与未处理的织物相比）。因此，开发的HAp纳米纤维涂层PLLA作为普通和整形外科领域的植入材料以及作为组织工程中的细胞支架具有可能的功效。(C)2009年，生物技术学会，日本。All rights reserved.
Cell adhesion and tissue response to poly(L-lactic acid) (PLLA) fabric coated with nanosized hydroxyapatite (HAp) crystals were studied. The HAp nanocrystals were prepared by the wet chemical process followed by calcination at 800 degrees C with an anti-sintering agent to prevent calcination-induced sintering. After the PLLA fabric was hydrolyzed with an alkaline aqueous solution, the HAp nanocrystals were coated via ionic interaction between the calcium ions on the HAp and the carboxyl groups on the alkali-treated PLLA. The PLLA surface uniformly coated with the HAp nanocrystals was observed by scanning electron microscope. The ionic interaction between the HAp and the PLLA was estimated by FT-IR. Improved cell adhesion to the HAp nanocrystal-coated surface was demonstrated by in vitro testing using a mouse fibroblast cell line L929. Furthermore, reduced inflammatory response to the HAp nanocrystal-coated PLLA fabric (as compared with a non-treated one) was confirmed by a subcutaneous implantation test with rats. Thus the HAp nanocrystal-coated PLLA developed has possible efficacy as an implant material in the fields of general and orthopedic surgery, and as a cell scaffold in tissue engineering. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.