Diversity and Abundance of the Denitrifying Microbiota in the Sediment of Eastern China Marginal Seas and the Impact of Environmental Factors
Diversity and Abundance of the Denitrifying Microbiota in the Sediment of Eastern China Marginal Seas and the Impact of Environmental Factors
复制标题
中国东部边缘海沉积物中反硝化微生物的多样性和丰富度及环境因素的影响
DOI：
10.1007/s00248-016-0906-6
复制
发表时间：
2017-04-01
影响因子：
3.6
通讯作者：
Zhang, Xiao-Hua
中科院分区：
文献类型：
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作者：
Gao, Minghong;Liu, Jiwen;Zhang, Xiao-Hua
研究海洋沉积物生态系统中对社区组成的环境影响和丹叶体的丰度对于理解生物地球化学过程的生态系统水平控制至关重要。在本研究中，通过克隆图书馆分析研究了中国东部边际海洋（ECMS）不同泥土矿床区域（ECMS）中不同泥土沉积区的nirk-harborring nitrify群落。通过荧光定量PCR评估了与非硝化相关的三个功能基因（Narg，Nirk，Nosz）。 Nirk-Harboring微生物群以一些操作分类单元（OTU）为主，它们在不同的地点分布在不同的地点，每个地点都有其独特的类型。 NIRK的平均丰度显着高于NARG和NOSZ基因，Narg的丰度高于NOSZ。沿着反硝化过程的不同功能基因的不一致的丰度可能表明，亚硝酸盐的减少是独立于ECMS的泥浆沉积区中的硝化的，而沉积的硝化作用是通过合作的不同非硝化物种而不是单个物种来实现的。仅靶向单个硝化功能基因时，将错过此类重要信息。对丰度比与环境因素之间的相关性分析表明，在不同的泥浆沉积区中，反硝化物对环境因素的响应不变。我们的结果表明，对不同反硝化功能基因的全面分析可能会获得有关海洋沉积物中硝化微生物群动力学的更多信息。
Investigating the environmental influence on the community composition and abundance of denitrifiers in marine sediment ecosystem is essential for understanding of the ecosystem-level controls on the biogeochemical process of denitrification. In the present study,nirK-harboring denitrifying communities in different mud deposit zones of eastern China marginal seas (ECMS) were investigated via clone library analysis. The abundance of three functional genes affiliated with denitrification (narG,nirK,nosZ) was assessed by fluorescent quantitative PCR. ThenirK-harboring microbiota were dominated by a few operational taxonomic units (OTUs), which were widely distributed in different sites with each site harboring their unique phylotypes. The mean abundance ofnirKwas significantly higher than that ofnarGandnosZgenes, and the abundance ofnarGwas higher than that ofnosZ. The inconsistent abundance profile of different functional genes along the process of denitrification might indicate that nitrite reduction occurred independently of denitrification in the mud deposit zones of ECMS, and sedimentary denitrification was accomplished by cooperation of different denitrifying species rather than a single species. Such important information would be missed when targeting only a single denitrifying functional gene. Analysis of correlation between abundance ratios and environmental factors revealed that the response of denitrifiers to environmental factors was not invariable in different mud deposit zones. Our results suggested that a comprehensive analysis of different denitrifying functional genes may gain more information about the dynamics of denitrifying microbiota in marine sediments.