Flocculating properties and potential of Halobacillus sp. strain H9 for the mitigation of Microcystis aeruginosa blooms.
Flocculating properties and potential of Halobacillus sp. strain H9 for the mitigation of Microcystis aeruginosa blooms.
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盐杆菌的絮凝特性和潜力。
DOI：
10.1016/j.chemosphere.2018.11.082
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Xu Hong
中科院分区：
文献类型：
--
作者：
Zhang Danyang;Ye Qian;Zhang Fuxing;Shao Xueping;Fan Yongxiang;Zhu Xiaoying;Li Yinan;Yao Luming;Tian Yun;Zheng Tianling;Xu Hong
铜绿微囊藻可以在世界范围内的淡水中引起有害藻华。它已经严重影响到人类的生活，并阻碍了水资源的使用。因此，迫切需要开发生态友好的有效方法来控制和消除水环境中的铜绿微囊藻。在本研究中，Halobacillussp.分离筛选出一株具有高絮凝活性的细菌H9，以评估其去除铜绿微囊藻的潜力。絮凝活性和絮凝方式的分析表明，菌株H9是通过分泌活性物质而不是直接接触藻细胞来诱导好氧微囊藻絮凝的。5%浓度的H9培养上清液可有效絮凝产气荚膜梭菌，密度可达5 × 107个/mL。zeta电位的急剧增加表明电中和可能是絮凝过程的机理。菌株H9絮凝铜绿微囊藻，对藻细胞膜无损伤，不会导致藻毒素释放到周围环境中。絮凝的藻类培养物对斑马鱼幼虫的毒性较小，表明H9上清液的环境友好益处。除了铜绿微囊藻，H9菌株还能够絮凝其他两种引起有害藻华的物种，球形棕囊藻和赤潮异弯藻。此外，H9上清液的絮凝活性在不同温度和宽pH范围内稳定。这些特性使H9菌株在减轻有害藻华的影响方面具有很大的潜力。
Microcystis aeruginosacan cause harmful algal blooms in freshwaters worldwide. It has already seriously affected human lives and prevented the use of water resources. Therefore, there is an urgent need to develop ecofriendly and effective methods to control and eliminateM. aeruginosain aquatic environments. In this study,Halobacillussp. strain H9, a bacterium that showed highM. aeruginosaflocculation activity, was isolated and selected to assess its potential for the removal ofM. aeruginosa. The analyses of flocculation activity and mode indicated that the strain H9 inducedM. aeruginosaflocculation by secreting active flocculating substance rather than by directly contacting algal cells. A 5% concentration of the H9 supernatant could efficiently flocculateM. aeruginosacells with a density of up to 5 × 107cells/mL. Dramatic increases in the zeta potential indicated that charge neutralization could be the mechanism of the flocculation process. The strain H9 flocculatedM. aeruginosawith no damage to the algal cell membrane, and did not result in microcystin being released into the surrounding environment. The flocculated algal culture was less toxic to zebrafish larvae, suggesting an environmentally friendly benefit of the H9 supernatant. In addition toM. aeruginosa,the H9 strain was also able to flocculate two other species causing harmful algal blooms,Phaeocystis globoseandHeterosigma akashiwo. Furthermore, the flocculation activity of the H9 supernatant was stable at different temperatures and over a wide pH range. These characteristics give the H9 strain great potential for mitigating the influences of harmful algal blooms.