METABOLIC BASIS TO FLUID PUMP IN CORNEA
METABOLIC BASIS TO FLUID PUMP IN CORNEA
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DOI：
10.1113/jphysiol.1972.sp009736
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发表时间：
1972-01-01
影响因子：
5.5
通讯作者：
MAURICE, DM
中科院分区：
文献类型：
--
作者：
DIKSTEIN, S;MAURICE, DM
1.本文介绍了一种在显微镜下连续测量兔角膜厚度的分离和灌注技术。研究了在37° C下用各种溶液灌注时冷肿胀角膜厚度的逆转。发现只有腺苷和谷胱甘肽的组合能够可靠地将厚度逆转至其正常值。该机制是钠、碳酸氢盐和氧依赖性的。糖酵解或电子传递的抑制剂停止逆转过程。这些发现与ATP酶的需要是一致的，但不能区分其调节的几种可能性。
1. A technique for isolating and perfusing the rabbit cornea is described, which allowed its thickness to be continuously measured under the microscope. The reversal of thickness of the cold‐swollen cornea on perfusion with various solutions at 37° C was studied.2. It was found that only a combination of adenosine and glutathione was able to reliably reverse the thickness to its normal value. This mechanism is sodium, bicarbonate, and oxygen dependent. Inhibitors of glycolysis or electron transport stop the reversal process.3. The findings are consistent with a need for an ATPase but are not capable of distinguishing among several possibilities for its regulation.