Radioimmunoassay for glycoprotein gp47 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera.
Radioimmunoassay for glycoprotein gp47 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera.
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放射免疫分析小鼠器官和血清中鼠乳腺肿瘤病毒糖蛋白gp47，并在人血清中寻找相关抗原。
作者：
P. F. Zangerie;C. Calberg‐Bacq;C. Colin;P. Franchimont;C. François;L. Gosselin;S. Kozma;P. Osterrieth
用二乙基氨乙基纤维素和羟基磷灰石层析法分离纯化小鼠乳腺肿瘤病毒主要糖蛋白（gp47）作为标记抗原，建立了特异性高、重复性好的放射免疫分析方法。病毒的其他七种（糖）蛋白与gp47抗原性不同。未感染的C57BL小鼠的血清和器官不含gp47，但感染的Swiss和RIII小鼠的血清中确实含有该抗原。尽管乳腺中的含量很高，但雄性和雌性小鼠其他器官中的gp47水平相同。血清中gp47的滴度在荷瘤雌性小鼠中较高，但其因品系而异。未检出抗gp47免疫球蛋白。本研究包括314份人血清（107份正常人血清、65份乳腺良性疾病血清、89份乳腺癌血清和53份消化道癌血清）。没有一个含有与gp47相关的抗原。20例人乳腺囊液中1例阳性。
Murine mammary tumor virus main glycoprotein (gp47), prepared by diethylaminoethyl cellulose and hydroxyapatite chromatography of detergent-mercaptoethanol-KCI-disrupted virion, was used as labeled antigen in a highly specific and reproducible radioimmunoassay. Seven other (glyco) proteins of the virus were antigenically distinct from gp47. Serum and organs of unifected C57BL mice did not contain gp47, but sera of infected Swiss and RIII mice did contain the antigen. Despite the high content in the mammary gland, the level of gp47 in other organs was identical in male and female mice. The titer of gp47 in serum was high in tumor-bearing females, but it varied with the mouse strain. Anti-gp47 immunoglobulins could not be detected. The investigation included 314 human sera (107 normal, 65 benign mastopathy, 89 breast vancer, and 53 digestive cancer). None contained an antigen related to gp47. One of 20 human mammary cyst fluids was positive.