Plasma free hemoglobin: A novel diagnostic test for assessment of the depth of burn injury
Plasma free hemoglobin: A novel diagnostic test for assessment of the depth of burn injury
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DOI：
10.1097/01.prs.0000200070.66604.1e
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Tan, KC
中科院分区：
文献类型：
--
作者：
Wong, CH;Song, C;Tan, KC
背景：准确确定烧伤深度是困难的，即使是经验丰富的外科医生。作者假设，血浆游离血红蛋白烧伤后的水平与烧伤的深度，他们评估了这一假设在一个小鼠model.Methods：全层和部分厚度烧伤不同大小的38和36只雄性Wistar大鼠，分别造成。结果：Ⅲ度烧伤引起的溶血是Ⅱ度烧伤的2倍（P <0.05），Ⅲ度烧伤引起的溶血是Ⅱ度烧伤的2倍（P <0.05），Ⅲ度烧伤引起的溶血是Ⅱ度烧伤的2倍（P < 0.05）。血浆游离血红蛋白水平与全身烧伤面积呈线性相关，其中Ⅲ度烧伤组（r = 0.91，p < 0.001）与Ⅱ度烧伤组（r = 0.94，p < 0.001）均呈线性相关。溶血量与烧伤总表面积的相关性在伤后15分钟最强。血浆游离血红蛋白在烧伤后15 ~ 30分钟迅速升高，随后下降。结论：烧伤后血浆游离血红蛋白水平与烧伤面积和深度有关。该测试可能是一个有价值的诊断辅助烧伤的评估。
Background: Accurate determination of the depth of burn injury is difficult, even for experienced surgeons. The authors hypothesized that the level of plasma free hemoglobin following burn injury is correlated to the depth of burn injury, and they evaluated this hypothesis in a murine model.Methods: Full-thickness and partial-thickness burn injuries of varying sizes were inflicted on 38 and 36 male Wistar rats, respectively. Blood specimens were taken at 0, 15, 30, 45, and 60 minutes after burn injury, and the levels of plasma free hemoglobin were determined spectrophotometrically.Results: Full-thickness burns cause two times more hemolysis than partial-thickness burns (p < 0.05). A linear correlation was demonstrated between plasma free hemoglobin levels and total body surface area burned in both the full-thickness (r = 0.91, p < 0.001) and partial-thickness burn groups (r = 0.94, p < 0.001). The correlation between the quantity of hemolysis and the total body surface area burned was strongest at 15 minutes after the onset of burn injury. The levels of free hemoglobin peaked rapidly between 15 and 30 minutes after thermal injury and declined thereafter.Conclusions: The authors' data suggest that the level of plasma free hemoglobin after burn injury is related to the size and depth of burn injury. This test can potentially be a valuable diagnostic adjunct in the assessment of burns.