One stomach one strain -: Does Helicobacter pylori strain variation influence disease outcome?
One stomach one strain -: Does Helicobacter pylori strain variation influence disease outcome?
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DOI：
10.1023/a:1026658301825
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发表时间：
1999-01-01
影响因子：
3.1
通讯作者：
Engstrand, L
中科院分区：
文献类型：
--
作者：
Enroth, H;Nyrén, O;Engstrand, L
这项研究的目的是基于基因组指纹和cagA(细胞毒素相关基因A)状态来确定幽门螺杆菌菌株的患者间和患者内的变异。从10例胃癌(GC)、10例十二指肠溃疡(DU)和10例胃炎(GI)患者中各采集10个细菌菌落。分析了可能的粘附素基因、cagA基因的存在，以及通过随机引物(AP)聚合酶链式反应(AP-PCR)获得的菌株特异性带型。基因组指纹图谱显示患者间存在广泛差异，但从同一患者的菌落获得的带型总是相同的(患者内差异)。在5名患者中，cagA状态在不同的菌落之间不同，尽管带型相同。在瑞典等发达国家的患者中，幽门螺杆菌多重菌株感染的比例很低，但菌株中存在不同cagA状态的亚克隆。
The aim of the study was to determine inter- and intrapatient variation of Helicobacter pylori strains based on genomic fingerprinting and cagA (cytotoxin-associated gene A) status. Ten bacterial colonies from each of 10 patients with gastric cancer (GC), 10 with duodenal ulcer (DU), and 10 with gastritis (GI) were used. The presence of the putative adhesin gene, the cagA gene, and the strain specific banding pattern obtained by arbitrary primed (AP-) PCR was analyzed. Genomic fingerprinting showed extensive interpatient variation, but the banding patterns obtained from colonies from the same patient were always identical (intrapatient variation). In five patients, the cagA status varied between the colonies despite identical banding patterns. Among patients in a developed country such as Sweden, the proportion with multiple-strain infection of H. pylori is low, but subclones with differing cagA status exist within the strain.