Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant
Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant
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DOI：
10.1007/s10526-007-9144-7
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发表时间：
2008-12
期刊：
影响因子：
2.5
通讯作者：
Jian-gang Li;Zhi-qiang Jiang;Liu-Ping Xu;Fei-Fei Sun-Fei;Jian-hua Guo
中科院分区：
文献类型：
--
作者：
Jian-gang Li;Zhi-qiang Jiang;Liu-Ping Xu;Fei-Fei Sun-Fei;Jian-hua Guo
从茄子根际分离得到353株分泌几丁质酶的菌株CH2。根据16S rDNA序列比对和多项生化生理特征，鉴定该菌株为蜡样芽孢杆菌。在几丁质层（CA）培养基上，菌株分泌几丁质酶。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴定，该酶为15.0 kd几丁质酶。在载玻片上，细菌悬浮液、悬浮液上清液和从菌株CH2中提取的0.005%几丁质酶溶液有效抑制了真菌孢子的萌发。几丁质酶的最适pH为7.1，最适温度为40℃。在该温度下，高水平的几丁质降解活性保持了10天。在温室试验中，CH2菌株的细胞悬浮液在14 d内使茄子黄萎病的严重程度降低了69.69%，上清液降低了54.04%，酶稀释到0.01%的强度降低了53.13%。菌株CH2及其几丁质酶在防治黄萎病方面具有良好的商业潜力。
A chitinase-secreting strain CH2 was one of 353 strains isolated from rhizosphere of eggplant. Based on 16S rDNA sequence alignment and several biochemical and physiological characteristics, the strain was identified to be ofBacillus cereus. On chitin–Ayers (CA) medium, the strain secreted chitinase. Evaluation of its activity, combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), showed it to be a 15.0-KD chitinase. On glass slides, germination of the fungal spores was effectively suppressed by the bacterial suspension, supernatant from the suspension, and 0.005% solution of chitinase extracted from the strain CH2. The optimum pH for chitinase was 7.1 and optimum temperature was 40°C. At that temperature, high-level chitinolytic activity was retained for 10 days. In greenhouse experiments, suspension of the cells of the CH2 strain reduced the severity of Verticillium wilt on eggplant by 69.69%, its supernatant by 54.04%, and the enzyme diluted to 0.01% strength by 53.13% in 14 days. Strain CH2 and its chitinase have good commercial potential in controlling Verticillium wilt.