Molecular characterisation of a novel cassava associated circular ssDNA virus
Molecular characterisation of a novel cassava associated circular ssDNA virus
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DOI：
10.1016/j.virusres.2012.03.009
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发表时间：
2012-06-01
期刊：
影响因子：
5
通讯作者：
Varsani, Arvind
中科院分区：
文献类型：
--
作者：
Dayaram, Anisha;Opong, Allen;Varsani, Arvind
环状单链（ss）DNA分子的序列非特异性滚环扩增在病毒宏基因组学中的应用促进了在各种生态系统中发现可能是多种多样的新型ssDNA病毒。在这里，我们描述了一个假定的新的ssDNA病毒（在基因组水平），木薯相关的环状DNA病毒（CasCV），从木薯叶样品感染的真菌Collectotrichum和Plectosphaerella。CasCV具有环状双义基因组，并且与核盘菌低致病性相关DNA病毒1（SsHADV-1）、藓类VEM病毒SDBVL和Meles梅特斯粪便病毒（MmFV）具有显著的基因组相似性。CasCV基因组（2220 nt）具有三个大的开放阅读框。虽然其中一个可能编码衣壳蛋白，但其他两个可能在去除内含子后表达复制相关蛋白（Rep），例如在一些双生病毒的Rep编码基因中发现的内含子。该Rep将包含先前在双生病毒、圆环病毒和纳米病毒Rep中鉴定的四个保守的滚环复制（RCR）相关基序。鉴于CasCV Rep和CP分别与SsHADV-1的Rep和CP共享62.7%和39.8%的氨基酸同一性，并且发现CasCV与木薯感染真菌相关，我们建议CasCV应归入真菌病毒分类学家族。然而，需要使用感染性克隆进行宿主范围研究，以证明新病毒的可能来源和实际宿主物种。(C)2012爱思唯尔有限公司版权所有。
The application of sequence non-specific rolling circle amplification of circular single stranded (ss) DNA molecules to viral metagenomics has facilitated the discovery in various ecosystems of what is probably a diverse array of novel ssDNA viruses. Here we describe a putative novel ssDNA virus (at a genome level), cassava associated circular DNA virus (CasCV), isolated from cassava leaf samples infected with the fungi Collectotrichum and Plectosphaerella. CasCV has a circular ambisense genome and shares significant genome similarities with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), Mosquito VEM virus SDBVL and Meles metes faecal virus (MmFV). The CasCV genome (2220 nt) has three large open reading frames. While it is probable that one of these encodes a capsid protein, the other two probably express a replication associated protein (Rep) following the removal of an intron such as that found in the Rep encoding genes of some geminiviruses. This Rep would contain four conserved rolling circle replication (RCR) related motifs that have previously been identified in geminivirus, circovirus and nanovirus Reps. Given both that the CasCV Rep and CP share 62.7% and 39.8% amino acid identity respectively with the Rep and CP of SsHADV-1, and that CasCV was discovered associated with cassava infecting fungi, we suggest that CasCV should be classified within the mycovirus taxonomic family. However, host range studies using infectious clones will be required to demonstrate the novel virus' likely origin and actual host species. (C) 2012 Elsevier B.V. All rights reserved.