DNA Framework-Based Topological Cell Sorters
DNA Framework-Based Topological Cell Sorters
复制标题
基于 DNA 框架的拓扑细胞分选仪
DOI：
10.1002/anie.202002020
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhu Ying
中科院分区：
文献类型：
--
作者：
Yin Fangfei;Li Min;Mao Xiuhai;Li Fan;Xiang Xuelin;Li Qian;Wang Lihua;Zuo Xiaolei;Fan Chunhai;Zhu Ying
在细胞生物学过程中，分子识别以配体和受体之间特定的锁-键相互作用为特征，这些配体和受体以拓扑聚类的形式普遍分布在细胞膜上。很少有拓扑工程配体系统能够探索配体-受体拓扑组织之间的结合强度。在这里，我们通过开发一个四面体DNA框架家族（tdf）来生成拓扑控制的配体，因此多个配体是化学计量和拓扑排列的。这种多配体的拓扑控制通过诱导受体聚类改变了分子识别的性质，因此结合强度显着提高（约10倍）。由tdf形成的拓扑复合物的精确工程很容易转化为有效的细胞图谱结合控制和细胞分选的细胞结合强度控制。这项工作为拓扑配体的多用途设计的发展铺平了道路。
Molecular recognition in cell biological process is characterized with specific locks‐and‐keys interactions between ligands and receptors, which are ubiquitously distributed on cell membrane with topological clustering. Few topologically‐engineered ligand systems enable the exploration of the binding strength between ligand‐receptor topological organization. Herein, we generate topologically controlled ligands by developing a family of tetrahedral DNA frameworks (TDFs), so the multiple ligands are stoichiometrically and topologically arranged. This topological control of multiple ligands changes the nature of the molecular recognition by inducing the receptor clustering, so the binding strength is significantly improved (ca. 10‐fold). The precise engineering of topological complexes formed by the TDFs are readily translated into effective binding control for cell patterning and binding strength control of cells for cell sorting. This work paves the way for the development of versatile design of topological ligands.