Prospects for safety testing: A strategy for the application of transgenic rodent mutagenesis assays
Prospects for safety testing: A strategy for the application of transgenic rodent mutagenesis assays
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DOI：
10.1002/(sici)1098-2280(1996)28:4
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发表时间：
1996
影响因子：
2.8
通讯作者：
N. Gorelick;J. Mirsalis
中科院分区：
文献类型：
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作者：
N. Gorelick;J. Mirsalis
在过去的几年中，转基因动物模型的开发和使用有了巨大的增长，以测量特定插入的报告基因的突变。这些系统提供了大量相关组织体内的基因突变数据。许多实验室现在都在使用这些系统，结果一致。本文描述了转基因诱变系统在产品开发和注册策略中可以填补的独特利基。除了组织特异性机制研究外，转基因检测还可用于跟踪沙门氏菌、大肠杆菌、小鼠淋巴瘤（L5178Y）细胞或其他体外系统中显示的诱变效应。©1996 Wiley‐Liss, Inc。
The past several years have seen an enormous increase in the development and use of transgenic animal models to measure mutations in specific inserted reporter genes. These systems provide gene mutation data in vivo in a wide range of relevant tissues. Numerous laboratories are now using these systems with consistent results. This paper describes the unique niche that transgenic mutagenesis systems can fill in product development and registration strategies. In addition to tissue‐specific mechanistic studies, transgenic assays are available to follow up mutagenic effects demonstrated in Salmonella, Escherichia coli, mouse lymphoma (L5178Y) cells, or other in vitro systems. © 1996 Wiley‐Liss, Inc.