Investigating the potential of Shikonin as a novel hypertrophic scar treatment.
Investigating the potential of Shikonin as a novel hypertrophic scar treatment.
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研究紫草素作为一种新型增生性疤痕治疗方法的潜力。
DOI：
10.1186/s12929-015-0172-9
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发表时间：
2015-08-16
影响因子：
11
通讯作者：
Upton Z
中科院分区：
文献类型：
--
作者：
Fan C;Xie Y;Dong Y;Su Y;Upton Z
增生性瘢痕形成是临床上非常普遍的病症，并且是由伤口愈合后瘢痕形成期间凋亡成纤维细胞数量减少和过度丰富的胶原蛋白产生引起的。我们前期的研究表明紫草素能诱导增生性瘢痕成纤维细胞凋亡，减少胶原的生成。在这里报道的研究中，我们通过使用Transwell®共培养技术检查紫草素对角质形成细胞和成纤维细胞的影响，进一步评估紫草素作为一种新型瘢痕修复疗法的潜在用途。并揭示了其内在机制。此外，还研究了紫草素对Transwell共培养条件培养基中细胞因子表达的影响。我们的研究结果表明，紫草素优先抑制细胞增殖，诱导成纤维细胞凋亡，而不影响角质形成细胞的功能。此外，我们还发现SHI的增殖抑制和凋亡诱导作用可能是通过MAPK和Bcl-2/Caspase 3信号通路实现的。此外，已发现SHI减弱Transwell共培养“条件”培养基中TGF-β1的表达。从这项研究中产生的数据提供了进一步的证据，支持紫草素作为一种新的疤痕修复治疗的潜在用途。
Hypertrophic scarring is a highly prevalent condition clinically and results from a decreased number of apoptotic fibroblasts and over-abundant production of collagen during scar formation following wound healing. Our previous studies indicated that Shikonin, an active component extracted from Radix Arnebiae, induces apoptosis and reduces collagen production in hypertrophic scar-derived fibroblasts. In the study reported here, we further evaluate the potential use of Shikonin as a novel scar remediation therapy by examining the effects of Shikonin on both keratinocytes and fibroblasts using Transwell® co-culture techniques. The underlying mechanisms were also revealed. In addition, effects of Shikonin on the expression of cytokines in Transwell co-culture “conditioned” medium were investigated. Our results indicate that Shikonin preferentially inhibits cell proliferation and induces apoptosis in fibroblasts without affecting keratinocyte function. In addition, we found that the proliferation-inhibiting and apoptosis-inducing abilities of SHI might be triggered via MAPK and Bcl-2/Caspase 3 signalling pathways. Furthermore, SHI has been found to attenuate the expression of TGF-β1 in Transwell co-cultured “conditioned” medium. The data generated from this study provides further evidence that supports the potential use of Shikonin as a novel scar remediation therapy.