Lack of Shiga-like toxin binding sites in germinal centres of mouse lymphoid tissues
Lack of Shiga-like toxin binding sites in germinal centres of mouse lymphoid tissues
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DOI：
10.1046/j.1365-2567.2002.01373.x
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发表时间：
2002-04-01
期刊：
影响因子：
6.4
通讯作者：
Kurohane, K
中科院分区：
文献类型：
--
作者：
Imai, Y;Fukui, T;Kurohane, K
生发中心的B细胞在人淋巴组织中表达碳水化合物抗原CD77。CD77抗原被肠出血性大肠杆菌O157:H7产生的志贺样毒素（SLTs）特异性识别。为了确定志贺样毒素-1 （SLT-1B）的结合亚基作为免疫原使用时是否会对小鼠免疫系统产生不良影响，我们研究了SLT-1B是否可以结合小鼠淋巴组织的生发中心。免疫小鼠外周血淋巴结冰冻切片和Peyer’s patches通过免疫组织学方法检测slt - 1b结合位点的存在。生发中心没有被SLT-1B染色，但被另一种生发中心标记物花生凝集素（PNA）强烈染色。另一方面，在小鼠肾脏冷冻切片中，SLT-1B特异性结合肾小管和收集管。这与人体组织的结果一致。我们还证明免疫小鼠淋巴结中的B220/PNA双阳性群体仅表现出SLT-1B的边缘染色。目前的结果表明，slt不会阻碍小鼠免疫系统生发中心的功能。
B cells in germinal centres are known to express carbohydrate antigen CD77 in human lymphoid tissues. The CD77 antigen is specifically recognized by Shiga-like toxins (SLTs) that are produced by enterohaemorrhagic Escherichia coli O157:H7. To determine whether the binding subunits of Shiga-like toxin-1 (SLT-1B) could have adverse effects on the murine immune system when used as an immunogen, we investigated whether SLT-1B could bind to germinal centres of mouse lymphoid tissues. Frozen sections of peripheral lymph nodes and Peyer's patches from immunized mice were tested for the presence of SLT-1B-binding sites by immunohistological methods. Germinal centres were not stained with SLT-1B, while they were intensely stained with peanut agglutinin (PNA), another marker of germinal centres. On the other hand, SLT-1B specifically bound to renal tubules and collecting ducts in frozen sections of mouse kidney. This is consistent with results from human tissues. We also demonstrated that B220/PNA double-positive populations in lymph nodes from immunized mice exhibited only marginal staining with SLT-1B. The present results suggest that SLTs would not impede germinal centre functions of the murine immune system.