CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
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CRISPR-Cas 生物化学和基于 CRISPR-的分子诊断
DOI：
10.1002/anie.202214987
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Weng, Zhengyan;You, Zheng;Yang, Jie;Mohammad, Noor;Lin, Mengshi;Wei, Qingshan;Gao, Xue;Zhang, Yi
基于聚合酶链反应（PCR）的核酸检测在疾病诊断、病原体监测等方面发挥了关键作用。然而，这种方法需要很长的周转时间，昂贵的设备和训练有素的人员，限制了其广泛的可用性和诊断能力。另一方面，成簇的规则间隔短回文重复序列（CRISPR）技术最近已经证明了具有高灵敏度和特异性的核酸检测能力。CRISPR介导的生物传感技术为彻底改变核酸检测程序和开发即时诊断提供了巨大的希望。本文重点介绍了基础CRISPR生物化学和基于CRISPR的核酸检测技术的最新进展。本书还涵盖了分子诊断领域的四个正在进行的研究热点--无靶向预扩增检测、microRNA（miRNA）检测、非核酸检测和SARS-CoV-2检测。
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR) technology has recently demonstrated capability for nucleic acid detection with high sensitivity and specificity. CRISPR‐mediated biosensing holds great promise for revolutionizing nucleic acid testing procedures and developing point‐of‐care diagnostics. This review focuses on recent developments in both fundamental CRISPR biochemistry and CRISPR‐based nucleic acid detection techniques. Four ongoing research hotspots in molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid detection, and SARS‐CoV‐2 detection‐are also covered.