Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent THI polarization
Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent THI polarization
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DOI：
10.1038/79747
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发表时间：
2000-10-01
影响因子：
30.5
通讯作者：
Colonna, M
中科院分区：
文献类型：
--
作者：
Cella, M;Facchetti, F;Colonna, M
浆细胞样树突状细胞（PDCs）是存在于人类血液和发炎淋巴结中的树突状细胞的子集。在这里，我们表明，血液PDCs，在体外用流感病毒和CD 40 L刺激时，经历了一个成熟的过程，其特征在于主要组织相容性复合体蛋白和粘附和共刺激分子的上调。此外，PDCs下调CXCR 3和L-选择素，其介导这些细胞迁移和归巢到淋巴结中。成熟的PDCs有效地刺激T细胞并在体外驱动有效的THI极化，这是由白细胞介素12和I型干扰素的协同作用介导的。在体内，成熟的PDCs存在于次级淋巴器官中，在那里它们代表炎症期间I型干扰素的主要来源。因此，PDCs可能参与抗病毒和促炎反应，而不是T（H）2极化和耐受诱导。
Plasmacytoid dendritic cells (PDCs) are a subset of dendritic cells present in human blood and inflamed lymph nodes. Here we show that blood PDCs, when stimulated with influenza virus and CD40L in vitro, undergo a maturation process characterized by up-regulation of major histocompatibility complex proteins and adhesion and costimulatory molecules. In addition, PDCs down-regulate CXCR3 and L-selectin, which mediate migration and homing of these cells into the lymph node. Mature PDCs efficiently stimulate T cells and drive a potent THI polarization in vitro, which is mediated by the synergistic effect of interleukin 12 and type I interferon. In vivo, mature PDCs are found in secondary lymphoid organs, where they represent the principal source of type I interferon during inflammation. Thus, PDCs probably participate in antiviral and pro-inflammatory responses, rather than in T(H)2 polarization and tolerance induction.