CYTOTYPE CONTROL OF DROSOPHILA-P ELEMENT TRANSPOSITION - THE 66 KD PROTEIN IS A REPRESSOR OF TRANSPOSASE ACTIVITY
CYTOTYPE CONTROL OF DROSOPHILA-P ELEMENT TRANSPOSITION - THE 66 KD PROTEIN IS A REPRESSOR OF TRANSPOSASE ACTIVITY
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DOI：
10.1016/0092-8674(90)90365-l
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发表时间：
1990-07-27
期刊：
影响因子：
64.5
通讯作者：
RIO, DC
中科院分区：
文献类型：
--
作者：
MISRA, S;RIO, DC
果蝇P转座因子编码两种蛋白，一种是87 kd的转座酶蛋白，另一种是66 kd的转座酶蛋白，据推测可以抑制转座。我们已经制作了携带仅编码66 kd蛋白的修饰P元件衍生物的种系转化子，并表明这些元件在种系和体细胞中都抑制转座酶活性。这些元件在基因组中的位置定量地影响了它们负调控转座酶和表达66 kd蛋白的能力。单个含66 kd元素的菌株没有表现出P株的细胞型特征的母系遗传。然而，我们证明了一个真正的P菌株在卵发生过程中产生了比单个66 kd P元素更高水平的66 kd蛋白。因此，在卵发生过程中，66kd抑制因子的表达可能是P细胞型母性效应的主要决定因素。
Drosophila P transposable elements encode two proteins, an 87 kd transposase protein and a 66 kd protein that has been hypothesized to repress transposition. We have made germline transformants carrying modified P element derivatives that encode only the 66 kd protein and shown that these elements repress transposase activity in both the germline and the soma. The position of these elements in the genome quantitatively affected their ability to negatively regulate transposase and to express the 66 kd protein. Single 66 kd element-containing strains did not exhibit the maternal inheritance of P cytotype characteristic of P strains. However, we demonstrated that a true P strain produced higher levels of the 66 kd protein during oogenesis than single 66 kd P elements. Thus, the expression of the 66 kd repressor during oogenesis may be a major determinant of the maternal effect of P cytotype.