32P-postlabeling analysis of liver DNA adducts in rats chronically fed a choline-devoid diet.
32P-postlabeling analysis of liver DNA adducts in rats chronically fed a choline-devoid diet.
复制标题
对长期饲喂无胆碱饮食的大鼠肝脏 DNA 加合物进行 32P 标记后分析。
DOI：
10.1093/carcin/8.1.187
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发表时间：
1987
期刊：
影响因子：
4.7
通讯作者：
Lombardi,B
中科院分区：
文献类型：
--
作者：
Gupta,RC;Earley,K;Locker,J;Lombardi,B
Liver DNA, obtained at various time intervals from rats chronically fed a choline-devoid diet, was analysed for the presence of aromatic or alkyl adducts by the32P-postlabeling assay. Alkyl adducts were not detected. Aromatic DNA adduct lesions were revealed, but only at levels (1 adduct per 0.5–3×109nucleotides) which are at the limits of the extremely high sensitivity of the method used, levels which remained constant throughout the period of feeding. Thus, contamination of the total environment of the animals with chemical carcinogens does not appear to be responsible for the genesis of the hepatocellular carcinomas that develop in rats chronically fed a choline-devoid diet. The diet, therefore, either acts as a complete carcinogen, or promotes the evolution to cancer of endogenous, ‘spontaneously’ initiated liver cells.
