The development of a highly informative mouse simple sequence length polymorphism (SSLP) marker set and construction of a mouse family tree using parsimony analysis
The development of a highly informative mouse simple sequence length polymorphism (SSLP) marker set and construction of a mouse family tree using parsimony analysis
复制标题
DOI：
10.1101/gr.717903
复制
发表时间：
2003-03-01
期刊：
影响因子：
7
通讯作者：
Brownstein, MJ
中科院分区：
文献类型：
--
作者：
Witmer, PD;Doheny, KF;Brownstein, MJ
为了为大量常用的小鼠杂交鉴定高信息量的标记，我们选择了现有小鼠简单序列长度多态性（SSLP）标记集的一个子集进行进一步开发。设计了314个SSLP标记的引物对，并针对54个自交系小鼠进行了分型。我们设计了新的PCR引物序列，用于使用荧光染料FAM， VIC， NED和ROX进行多路复用。C57BL/6J × DBA/2J的信息标记数为217个，平均间距为6.8 cM。其他菌株杂交信息标记数平均为197.0个（SD 37.8），标记间平均距离为6.8 cM （SD 1.1）。为了确定224个小家鼠和小家鼠之间多态性标记的定位，我们使用了Jackson实验室（TL）种间回交图谱（TIL BSS）；这些标记中有168个（75%）以前没有被其他研究者在这个杂交中绘制过，这为这个社区地图资源增加了新的信息。有了这个庞大的数据集，我们试图用瓦格纳简约分析重建实验室小鼠的系统发育历史。我们的结果在很大程度上与已知的近亲繁殖小鼠株的历史一致。
To identify highly informative markers for a large number of commonly employed murine crosses, we selected a subset of the extant mouse simple sequence length polymorphism (SSLP) marker set for further development. Primer pairs for 314 SSLP markers were designed and typed against 54 inbred mouse strains. We designed new PCR primer sequences for the markers selected for multiplexing using the fluorescent dyes FAM, VIC, NED, and ROX. The number of informative markers for C57BL/6J x DBA/2J is 217, with an average spacing of 6.8 centiMorgans (cM). For all other pairs of strains, the mean number of informative markers per cross is 197.0 (SD 37.8) with a mean distance between markers of 6.8 cM (SD 1.1). To confirm map positions of the 224 markers in our set that are polymorphic between Mus musculus and Mus spretus, we used The Jackson Laboratory (TL) interspecific backcross mapping panel (TIL BSS); 168 (75%) of these markers had not been previously mapped in this cross by other investigators, adding new information to this community map resource. With this large data set, we sought to reconstruct a phylogenetic history of the laboratory mouse using Wagner parsimony analysis. Our results are largely congruent with the known history of inbred mouse strains.