Identification of a Novel Polymorphism in Bovine lncRNA ADNCR Gene and Its Association with Growth Traits
Identification of a Novel Polymorphism in Bovine lncRNA ADNCR Gene and Its Association with Growth Traits
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牛lncRNA ADNCR基因新多态性的鉴定及其与生长性状的关联
DOI：
10.1080/10495398.2018.1456446
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发表时间：
2019-04-03
影响因子：
3.7
通讯作者：
Lan, Xianyong
中科院分区：
文献类型：
--
作者：
Jin, Yunyun;Yang, Qing;Lan, Xianyong
脂肪细胞分化相关长链非编码RNA（ADNCR）是一种新发现的lncRNA。它通过靶向miR-204在mRNA和蛋白质水平上显著调节前脂肪细胞中靶SIRT 1基因的表达，从而抑制脂肪形成。四引物扩增难治突变系统PCR（T-ARMS-PCR）策略在大样本SNP基因分型中是快速和准确的，成本可以忽略不计。本研究在牛ADNCR基因内含子1中发现了一个新的SNP g.1263T>A，应用T-ARMS-PCR方法对7个牛品种的1017个个体进行了牛ADNCR基因新SNP的基因型检测，并对3种不同基因型的90头牛进行了DNA测序，验证了该方法的准确性。两种方法之间的一致性为100%。关联分析表明，该位点与晋南牛体重（P = 0.010）、胸围（P = 0.014）、臀长（P = 0.038）、秦川牛髋骨宽（P = 0.032）、晋江牛管围（P = 0.019）显著相关。这些新的发现可能用于标记辅助选择（MAS），并有助于在未来的肉牛的性能。
ABSTRACT Adipocyte differentiation-associated long noncoding RNA (ADNCR) is a newly discovered lncRNA. It plays function by targeting miR-204 to significantly regulates the expression of the target SIRT1 gene in preadipocytes both at the level of mRNA and protein, thereby inhibiting adipogenesis. The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) strategy is fast and accuracy at a negligible cost for SNP genotyping in large samples. In the study, a novel SNP g.1263T>A in intron 1 of bovine ADNCR gene was found. Herein, the T-ARMS-PCR assay was applied to detect the genotypes of the novel SNP of bovine ADNCR gene in 1017 individuals from seven cattle breeds and validated the accuracy by DNA sequencing assay of ninety animals representing three different genotypes. The concordance between two different methods was 100%. The association analysis indicated that this locus was significantly associated with the body weight (P = 0.010), chest girth (P = 0.014) and rump length (P = 0.038) in Jinnan cattle, hucklebone width (P = 0.032) in Qinchuan cattle, the cannon circumference (P = 0.019) in Jinjiang cattle, respectively. These novel findings may be used for marker-assisted selection (MAS) and contribute to the performance of beef cattle in the future.