The effect of ferrous-chelating hairtail peptides on iron deficiency and intestinal flora in rats
The effect of ferrous-chelating hairtail peptides on iron deficiency and intestinal flora in rats
复制标题
亚铁螯合带鱼肽对缺铁大鼠及肠道菌群的影响
DOI：
10.1002/jsfa.7452
复制
发表时间：
2016-06-01
影响因子：
4.1
通讯作者：
Song, Ru
中科院分区：
文献类型：
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作者：
Lin, Hui-Min;Deng, Shang-Gui;Song, Ru
背景：螯合剂，例如小肽，可以降低游离铁含量并增加铁的生物利用度。它们可能具有良好的治疗潜力，并可能阻止低分子量铁的促氧化作用。带鱼是一种富含易消化蛋白质的鱼类。我们通过使用带鱼酶解产物作为螯合剂扩展了这种铁递送策略，发现亚铁螯合带鱼肽对贫血 Sprague-Dawley 大鼠具有抗贫血活性。 结果：在大鼠模型中研究了通过带鱼酶解产物和亚铁螯合制备的亚铁螯合带鱼肽的抗贫血活性 缺铁性贫血。 35天实验结束后，我们注意到补充亚铁螯合带鱼肽和FeSO4的动物与健康动物之间的血红蛋白、平均红细胞体积、血红蛋白分布宽度和铁蛋白浓度存在显着差异。对动物的生长或行为没有负面影响。肠粘膜固有层未见明显炎症，肠道菌群未见失衡。结论：新型亚铁螯合带鱼肽可能是改善缺铁状态的合适强化剂。我们的研究结果表明，这种多示踪剂技术在营养研究中具有许多应用。 (c) 2015年化学工业学会
BACKGROUND: Chelating agents, such as small peptides, can decrease free iron content and increase iron bioavailability. They may have promising therapeutic potential and may prevent the pro-oxidant effects of low molecular weight iron. Hairtail is a species of fish that is rich in easily digestible proteins. We extended this strategy for iron delivery by using an enzymatic hydrolysate of hairtail as the chelating agent and found that the ferrous-chelating hairtail peptides have anti-anaemic activity in Sprague-Dawley rats with anaemia.RESULTS: The anti-anaemic activity of ferrous-chelating hairtail peptides prepared by enzymatic hydrolysis of the hairtail and ferrous chelation was studied in rat models of iron deficiency anaemia. After the end of the 35 d experiment, we noted significant differences in haemoglobin, mean corpuscular volume, haemoglobin distribution width, and ferritin concentrations between those animals supplemented with ferrous-chelating hairtail peptides and FeSO4 and healthy animals. There were no negative side effects on the animals' growth or behaviour. There was no obvious inflammation in the intestinal mucosa lamina propria and no unbalance of intestinal flora.CONCLUSION: The novel ferrous-chelating hairtail peptides may be a suitable fortificant for improving iron-deficiency status. Our findings demonstrated that this multi-tracer technique has many applications in nutritional research. (c) 2015 Society of Chemical Industry