GROWTH IN SUCKLING-MOUSE BRAIN OF IBV-LIKE VIRUSES FROM PATIENTS WITH UPPER RESPIRATORY TRACT DISEASE
GROWTH IN SUCKLING-MOUSE BRAIN OF IBV-LIKE VIRUSES FROM PATIENTS WITH UPPER RESPIRATORY TRACT DISEASE
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DOI：
10.1073/pnas.58.6.2268
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
CHANOCK, RM
中科院分区：
文献类型：
--
作者：
MCINTOSH, K;BECKER, WB;CHANOCK, RM
利用人胚胎气管器官培养，在本实验室从感冒患者中分离到6株与禽传染性支气管炎病毒（JBV）形态相似的中型（120-160 mgt）病毒。这些病毒是在收集的器官培养液浓缩十倍并用电子显微镜检查时发现的。在阴性染色的制剂中，病毒呈圆形和中等多形性，具有间距广泛的棒状表面突出。这类病毒的形态与黏液病毒明显不同，因此暂定为“ibv样”病毒。“ibv样”病毒被乙醚灭活，但其生长不受5-溴脱氧尿苷的抑制。器官培养的血清学研究表明，从病毒中恢复的患者产生了中和抗体反应。然而，由于缺乏支持其生长的组织培养或实验动物系统，对这些病毒的进一步研究受到阻碍。由于这个原因，并且由于我们发现小鼠肝炎病毒（MHV），另一种含有rna的醚不稳定病毒，在阴性染色制剂中与“ibv样”病毒组成员在形态上相同，因此我们给2只新生小鼠接种了“ibv样”病毒株。小鼠接种6株病毒中的2株后可发生脑炎。这里报告了这些研究的初步结果。“ibv样”病毒在哺乳小鼠脑内的生长-“ibv样”病毒最初是通过将感冒患者的洗喉液接种到人类胚胎气管器官培养物中来恢复的。用于小鼠接种的悬浮液由器官培养中传代4至5次的材料制备而成，在10倍浓度后通过电子显微镜检查含有“ibv样”病毒颗粒。CD-1株的怀孕瑞士小鼠来自Charles River 1\louse Farms， Inc., Wilmington,:\Mass。几年前，来自这个群体的老鼠被证明没有感染ihv。”在我们的研究中，我们无法证明在实验室接受的小鼠中存在MHV抗体或感染。这些老鼠被放在电池罐中，盖子上插入了一个紧密贴合的3/8英寸玻璃纤维材料垫。严格的隔离措施被观察到，老鼠被安置在一个只用于这些实验的房间里。采用颅内（IC）和腹腔（IP）联合接种或仅通过颅内（IC）途径进行乳鼠传代。在第一次传代中，六个菌株中的两个，命名为OC38和OC43，在接种后11-15天开始引起疾病，其特征是全身震颤、僵硬和嗜睡。接种OC38的8只小鼠中有2只感染，接种OC43的16只小鼠中有3只感染。与此同时，16只未接种的对照组小鼠保持良好状态。在脑悬液的连续亚代中，综合症2268
With the use of humanembryonic tracheal organ culture, 9 six strains of a medium-sized (120-160 mgt) virus bearing a close morphologic resemblance to avian infectious bronchitis virus (JBV) were recovered in this laboratory from patients with colds. 13 These viruses were detected when organ culture fluid harvests were concentrated ten times and examined with the electron microscope. In negatively stained preparations the viruses appeared round and moderately pleomorphic with widely spaced, club-shaped surface projections. The tentative designation of" IBV-like" viruses was given to members of this group, whose morphology clearly differed from that of the myxoviruses. The" IBV-like" viruses were shown to be inactivated by ether, but their growth was not inhibited by 5-bromodeoxyuridine. 12 Serologic studies in organ culture suggested that the patients from whom the viruses were recovered developed a neutralizingantibody response. However, further study of these viruses was impeded by the lackof a tissue culture or experimental animal system which would support their growth. For this reason, and because we found that mouse hepatitis virus (MHV), another RNA-containing ether-labile virus, was morphologically identical to mem-bers of the" IBV-like" virus group in negatively stained preparations, 2 newborn mice were inoculated with the" IBV-like" virus strains. Encephalitis occurred in mice inoculated with two of the six strains. The preliminary resultsof these studies are reported here.Growth of" IBV-Like" Viruses in Suckling-Mouse Brain.-" IBV-like" viruses were originally recovered by inoculating throat washings from patients with colds onto human embryonic tracheal organ cultures. Suspensions usedfor mouse in-oculation were prepared from material passaged four to five times in organ culture and contained" IBV-like" virus particles when examined by electron microscopy after tenfold concentration. Pregnant Swiss mice of the CD-1 strainwere obtained from Charles River 1\louse Farms, Inc., Wilmington,:\Mass. Several years previously, the mice from this colonyhad been shown to be free of\_IHV contamination." 6 During our studies we were unable to demonstrate the presence of MHV antibody or infection in mice at the time they were received in the laboratory. The mice were kept in battery jars with a tightly fitting pad of 3/8-inch glass-fiber material inserted in the lid. Strict isolation precautions were observed, and the mice were housed in a room used only for these experiments. Suckling mouse passages were performed either by combined intracranial (IC) and intraperitoneal (IP) inoculation or by the IC route alone. On first passage, two of the six strains, designated OC38 and OC43, caused a disease which began 11-15 days after inoculation and was characterized by generalized tremors, rigidity, and lethargy. Two of 8 mice inoculated with OC38, and 3 of 16 mice inoculated with OC43 were affected. At the same time 16 control uninoculated mice remained well. On serial subpassage of brain suspensions, the syndrome 2268