Breakdown of distyly in a tetraploid variety of Ophiorrhiza japonica (Rubiaceae) and its phylogenetic analysis
Breakdown of distyly in a tetraploid variety of Ophiorrhiza japonica (Rubiaceae) and its phylogenetic analysis
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DOI：
10.1007/s10265-007-0089-9
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发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Yokota, Masatsugu
中科院分区：
文献类型：
--
作者：
Nakamura, Koh;Denda, Tetsuo;Yokota, Masatsugu
本文研究了四倍体蛇根草的花形态。阿马米亚娜·哈图斯和二倍体O.日本变种japonica的染色体，以阐明二倍性和倍性水平的关联。通过构建叶绿体DNA系统发育树，研究了四倍化事件和花形态转变的发生次数。日本。O.日本变种amamiana是长花柱的，而O.日本变种japonica为二型花，具有典型的长花柱和短花柱。花柱距离与倍性水平有关。套袋处理结果表明，O.日本变种amamiana是自交亲和的，并且可能自动自花授粉。在cpDNA测序分析中，两个品种之间没有共享的单倍型。单倍型网络显示O.日本变种amamiana，表明这一品种的单一起源。因此，O.的四倍体化和二分体到同株的分解都是可能的。日本变种阿马米亚纳可能只发生过一次。因为O.日本变种amamiana具有形态学和细胞学实体，被认为是一个单一的谱系，并与O.日本变种日本，这个变种可以被认为是一个独特的种。因此，我们建议提高O。日本变种amamiana到物种的等级。
We examined the floral morph of tetraploid Ophiorrhiza japonica Blume var. amamiana Hatus. and diploid O. japonica var. japonica to elucidate the association of distyly and ploidy levels. Chloroplast DNA phylogeny was reconstructed to determine the number of tetraploidization events and floral morph shifts in O. japonica. All individuals of O. japonica var. amamiana proved to be long-homostylous, whereas O. japonica var. japonica was distylous with typical long- and short-styled flowers. Distyly is related to the ploidy level. The bagging treatment of flowers indicated that O. japonica var. amamiana is self-compatible and potentially automatically self-pollinating. In cpDNA sequencing analysis, no haplotype was shared between the two varieties. The cpDNA haplotype network displayed the monophyly of O. japonica var. amamiana, suggesting a single origin of this variety. Hence, both tetraploidization and the breakdown of distyly to homostyly in O. japonica var. amamiana likely occurred just once. Because O. japonica var. amamiana having the morphological and cytological entity is recognized as a single lineage and clearly separated from O. japonica var. japonica, this variety can be considered to be a distinct species. We therefore propose to raise O. japonica var. amamiana to the rank of species.