Reduced genetic variation of an Onchocerca volvulus ABC transporter gene following treatment with ivermectin
Reduced genetic variation of an Onchocerca volvulus ABC transporter gene following treatment with ivermectin
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DOI：
10.1016/j.trstmh.2005.03.019
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Prichard, R. K.
中科院分区：
文献类型：
--
作者：
Ardelli, B. F.;Prichard, R. K.
在一些毛圆线虫中，伊维菌素 (IVM) 抗性的早期阶段的特点是等位基因频率的变化以及 P-糖蛋白基因、谷氨酸门控氯离子通道基因和 γ-氨基丁酸受体基因位点多态性的减少。 IVM 集体治疗是盘尾丝虫病控制计划的一个组成部分。对来自非洲几个种群的盘尾丝虫 ABC 转运蛋白同源物 (OvABC-3) 的遗传变异进行了检查，以确定该基因的等位基因与 IVM 治疗之间是否存在关联。来自加纳的未处理群体的等位基因变异表明该基因座具有高度多态性。然而，IVM 治疗人群的变异性有所降低。多晶型频率的 chi(2) 分析显示 1999 年在加纳收集的未经处理和处理过的样品之间存在显着差异。与 1999 年的样品相比，2002 年收集的样品中该基因的变异性较小。在一些接受治疗的人群中，似乎对 OvABC-3-C 进行了选择。在控制计划中观察到的变异性减少是可以预期的，经过多年的病媒控制和 IVM 分发后，感染的流行率和强度显着降低。多态性的减少本身可能并不表明这些盘旋丝虫具有 IVM 抗性，尽管它可能表明正在发生抗性选择。 (C) 2007 年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
In some trichostrongyloid nematodes, the early stages of ivermectin (IVM) resistance have been characterized by a shift in allele frequency and reduced polymorphism at loci of P-glycoprotein genes, glutamate-gated chloride channel genes and gamma-aminobutyric acid receptor genes. Mass treatment with IVM is an integral component of the onchocerciasis control programmes. Genetic variation of an Onchocerca volvulus ABC transporter homologue (OvABC-3) from several populations in Africa was examined to determine whether an association exists between alleles of this gene and IVM treatment. Allelic variation in a non-treated population from Ghana showed this locus to be highly polymorphic. However, variability was reduced in IVM-treated populations. chi(2) analysis of polymorph frequencies showed significant differences between untreated and treated samples collected in Ghana in 1999. There was less variability in this gene in samples collected in 2002 compared with the 1999 samples. In some treated populations, there appeared to be selection on OvABC-3-C. The observed reduction in variability could be expected in a control programme in which prevalence and intensity of infections are markedly reduced after years of vector control and IVM distribution. The reduction in polymorphism may not in itself indicate that these O. volvulus are IVM resistant, although it could indicate that selection for resistance is occurring. (C) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.