Effect of bovine leukemia virus (BLV) infection on bovine mammary epithelial cells RNA-seq transcriptome profile
Effect of bovine leukemia virus (BLV) infection on bovine mammary epithelial cells RNA-seq transcriptome profile
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DOI：
10.1371/journal.pone.0234939
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发表时间：
2020-06-24
期刊：
影响因子：
3.7
通讯作者：
Ceriani, Maria Carolina
中科院分区：
文献类型：
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作者：
Cuesta, Lucia Martinez;Liron, Juan Pedro;Ceriani, Maria Carolina
牛白血病病毒（BLV）是一种引起牛流行性白血病（EBL）的δ-逆转录病毒，EBL是一种影响牛的淋巴组织增生性疾病。该病毒导致免疫系统失调，有利于继发感染的发展。在这种情况下，认为BLV感染牛的乳腺炎发病率增加。本研究的目的是分析BLV感染的乳腺上皮细胞系（MAC-T）的转录组谱。我们的研究结果表明，BLV感染MAC-T细胞的IFN I信号通路和参与防御反应的基因，以及胶原蛋白分解代谢过程和一些原癌基因和肿瘤抑制基因的表达改变。本研究结果为进一步了解BLV对牛乳腺上皮细胞免疫应答的影响提供了依据。
Bovine leukemia virus (BLV) is a delta-retrovirus responsible for Enzootic Bovine Leukosis (EBL), a lymphoproliferative disease that affects cattle. The virus causes immune system deregulation, favoring the development of secondary infections. In that context, mastitis incidence is believed to be increased in BLV infected cattle. The aim of this study was to analyze the transcriptome profile of a BLV infected mammary epithelial cell line (MAC-T). Our results show that BLV infected MAC-T cells have an altered expression of IFN I signal pathway and genes involved in defense response to virus, as well as a collagen catabolic process and some protooncogenes and tumor suppressor genes. Our results provide evidence to better understand the effect of BLV on bovine mammary epithelial cell's immune response.