Toxicity of silica nanoparticles depends on size, dose, and cell type
Toxicity of silica nanoparticles depends on size, dose, and cell type
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DOI：
10.1016/j.nano.2015.03.004
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发表时间：
2015-08-01
影响因子：
5.4
通讯作者：
Kim Kevin, Kyekyoon
中科院分区：
文献类型：
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作者：
Kim, In-Yong;Joachim, Elizabeth;Kim Kevin, Kyekyoon
采用直径为20-200 nm的单分散球形二氧化硅纳米颗粒（SNP）研究A549和HepG 2上皮细胞和NIH/3 T3成纤维细胞中的大小、剂量和细胞类型依赖性细胞毒性。这些精确控制大小的统一SNP消除了混合大小产生的不确定性，并且独特地允许完全依赖于大小的效应的探测。研究了细胞活力、膜破裂、氧化应激和细胞摄取。SNP细胞毒性的程度和机制被发现不仅是大小和剂量依赖性的，而且高度依赖于细胞类型。此外，与测试的其他尺寸的颗粒相比，60 nm SNP表现出非常不寻常的行为，这意味着使用纳米颗粒控制细胞活性的有趣的可能性。具体而言，60 nm SNP优先被细胞内吞，并且在高剂量下导致细胞活力不成比例地降低。目前的工作可能有助于阐明纳米颗粒诱导的cytotoxicity.From临床编辑现有的结果之间的某些矛盾：二氧化硅纳米颗粒正在研究中的许多研究领域，其在临床应用中的使用。尽管如此，颗粒大小和潜在毒性之间的关系仍有待阐明。在这篇文章中，作者研究了精确直径在20到200 nm之间的球形SNP对三种不同细胞类型的生物学效应，他们的结果应该为更好的药物设计提供更多的安全性数据。(C)2015 Elsevier Inc. All rights reserved.
Monodisperse spherical silica nanoparticles (SNPs) with diameters of 20-200 nm were employed to study size, dose, and cell-type dependent cytotoxicity in A549 and HepG2 epithelial cells and NIH/3T3 fibroblasts. These uniform SNPs of precisely controlled sizes eliminated uncertainties arising from mixed sizes, and uniquely allowed the probing of effects entirely size-dependent. Cell viability, membrane disruption, oxidative stress, and cellular uptake were studied. The extent and mechanism of SNP cytotoxicity were found to be not only size and dose dependent, but also highly cell type dependent. Furthermore, the 60 nm SNPs exhibited highly unusual behavior in comparison to particles of other sizes tested, implying interesting possibilities for controlling cellular activities using nanoparticles. Specifically, the 60 nm SNPs were preferentially endocytosed by cells and, at high doses, caused a disproportionate decrease in cell viability. The present work may help elucidate certain contradictions among existing results on nanoparticle-induced cytotoxicity.From the Clinical Editor: Silica nanoparticles are being investigated in many research areas for their use in clinical applications. Nonetheless, the relationship between particle size and potential toxicity remains to be elucidated. In this article, the authors studied the biological effects of spherical SNPs with precise diameters between 20 and 200 nm on three different cell types and their results should provide more data on safety for better drug design. (C) 2015 Elsevier Inc. All rights reserved.