Screening, identification of antarctic cold-adapted petroleum hydrocarbon-degrading bacteria and low-temperature degrading adaptation analysis.
Screening, identification of antarctic cold-adapted petroleum hydrocarbon-degrading bacteria and low-temperature degrading adaptation analysis.
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发表时间：
2010
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通讯作者：
Liu Fangming;Miao Jinlai;Dong Chunxia;Wang Yi-bin;Zang Jia-ye
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作者：
Liu Fangming;Miao Jinlai;Dong Chunxia;Wang Yi-bin;Zang Jia-ye
南极低温微生物资源丰富，低温降解菌的获得一直是国外学者关注的焦点。采用2216 E与MMC培养相结合的方法，从南极海域中筛选出石油烃降解冷适应菌。对4株南极冷适应降解菌的降解效率、16 S rRNA基因进行了分子鉴定，并基于16 S rRNA基因构建了系统发育树。选择南极冷适应降解菌NJ 49进行冷适应性研究。结果表明，在以柴油为唯一碳源和能源的培养基中，有3株海洋细菌冷适应菌（NJ 41、NJ 49、NJ 289）生长良好。16 S rRNA鉴定结果表明，南极低温降解菌NJ 41、NJ 49和NJ 289分别属于Planococcus属、Shewanella属和Pseudo-alteromonas属。脂肪酸的变化表明，南极低温降解菌NJ 49可能是通过改变脂肪酸的膜修饰来适应低温条件进行降解的。
Low temperature microorganism resources of Antarctica were abundant and the scholar abroad have focused on obtaining cold-adapted degrading bacteria. The method of combination 2216E with MMC culture was used for screen- ing petroleum hydrocarbon-degrading cold-adapted bacteria which were from Antarctic seawaters . Degradation efficient, molecular identification of 16S rRNA amplified from four Antarctic cold-adapted degrading bacteria and phylogenetic tree based on 16S rRNA gene was carried. Antarctic cold-adapted degrading bacteria NJ49 was chosen to study cold adaptation. The results suggested that three marine bacteria cold-adapted bacteria (namely NJ41, NJ49, NJ289) grew well in the cul- ture which diesel was the sole carbon source and energy source. Results of 16S rRNA identification revealed that Antarctic cold-adapted degrading bacteria NJ41, NJ49 and NJ289 belonged to the described genus Planococcus, Shewanella, Pseudo-alteromonas, respectively. Data of fatty acid change revealed that the Antarctic cold-adapted degrading bacteria NJ49 maybe adapt cold condition to degrade by membrane modification of changing fatty acid.