SALMONELLA INDUCES THE FORMATION OF FILAMENTOUS STRUCTURES CONTAINING LYSOSOMAL MEMBRANE-GLYCOPROTEINS IN EPITHELIAL-CELLS
SALMONELLA INDUCES THE FORMATION OF FILAMENTOUS STRUCTURES CONTAINING LYSOSOMAL MEMBRANE-GLYCOPROTEINS IN EPITHELIAL-CELLS
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DOI：
10.1073/pnas.90.22.10544
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发表时间：
1993-11-15
影响因子：
11.1
通讯作者：
FINLAY, BB
中科院分区：
文献类型：
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作者：
GARCIADELPORTILLO, F;ZWICK, MB;FINLAY, BB
沙门氏菌在膜结合的空泡中的上皮细胞内侵入和复制。在这份报告中，我们表明，感染后的HeLa上皮细胞，鼠伤寒沙门氏菌驻留在空泡中含有溶酶体膜糖蛋白（lgps）。侵入后4 - 6小时，胞内细菌诱导形成含有lgps的稳定丝状结构，其连接到含细菌的空泡。这些富含lgp的结构的形成需要活的细胞内细菌，并且被液泡酸化的抑制剂阻断。这些结构不存在于未感染的细胞或感染另一种侵袭性细菌小肠结肠炎耶尔森氏菌的细胞中。添加到细胞外介质的示踪剂不被递送到沙门氏菌诱导的丝，这表明这些结构不同于先前描述的管状溶酶体。细胞内细菌复制的起始与这些含lgp的丝状体的形成相关。某些细胞内复制缺陷的无毒沙门氏菌突变体不能诱导这些结构的形成。这些观察结果表明，沙门氏菌诱导的丝含有lgps与细胞内细菌复制。
Salmonella species invade and replicate within epithelial cells in membrane-bound vacuoles. In this report we show that upon infection of HeLa epithelial cells, Salmonella typhimurium resides in vacuoles that contain lysosomal membrane glycoproteins (lgps). Four to six hours after invasion, intracellular bacteria induce the formation of stable filamentous structures containing lgps that are connected to the bacteria-containing vacuoles. Formation of these lgp-rich structures requires viable intracellular bacteria and is blocked by inhibitors of vacuolar acidification. These structures are not present in uninfected cells or in cells infected with another invasive bacteria, Yersinia enterocolitica. Tracers added to the extracellular medium are not delivered to the Salmonella-induced filaments, suggesting that these structures are different from previously described tubular lysosomes. Initiation of intracellular bacterial replication correlates with formation of these lgp-containing filaments. Certain avirulent Salmonella mutants that are defective for intracellular replication fail to induce formation of these structures. These observations suggest that Salmonella-induced filaments containing lgps are linked to intracellular bacterial replication.