Bcl-2-functionalized ultrasmall superparamagnetic iron oxide nanoparticles coated with amphiphilic polymer enhance the labeling efficiency of islets for detection by magnetic resonance imaging.
Bcl-2-functionalized ultrasmall superparamagnetic iron oxide nanoparticles coated with amphiphilic polymer enhance the labeling efficiency of islets for detection by magnetic resonance imaging.
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两亲性聚合物包被的 Bcl-2 功能化超小超顺磁性氧化铁纳米粒子提高了磁共振成像检测胰岛的标记效率
DOI：
10.2147/ijn.s52058
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发表时间：
2013
影响因子：
8
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Yang B;Cai H;Qin W;Zhang B;Zhai C;Jiang B;Wu Y
超顺磁性氧化铁（SPIO）或超小超顺磁性氧化铁（USPIO）纳米颗粒基于其通用的生物相容性，用于检测和追踪体内细胞或肿瘤。在这里，我们开发了一种新颖的B细胞淋巴瘤（Bcl）-2单克隆抗体功能化的USPIO纳米颗粒的合成方法，该纳米颗粒涂覆有两亲性聚合物（羧化聚乙二醇单油基醚[OE-PEG-COOH]）。这些纳米颗粒可以在相对低的铁浓度下被β细胞有效地内化并标记原代胰岛细胞。通过与市售USPIO产品FeraSpin™ S进行比较，研究了这些产品的生物相容性和细胞毒性。我们还评估了产品的安全剂量范围。虽然一些病例在移植部位显示出低信号变化，但在体内，在3.0特斯拉的场强下，通过临床MRI扫描仪可检测到强磁共振成像（MRI），并且通过普鲁士蓝和免疫组织化学染色证实了胰岛中的铁沉积/附着。值得注意的是，基于我们的合成方法，将来我们可以将Bcl-2与其他探针交换，这些探针对靶细胞更具特异性，并且在体内具有更好的标记特异性。综合结果表明，新型Bcl-2功能化PEG-USPIO作为胰岛细胞或其他细胞体内监测的分子成像剂具有很大的潜力。
Based on their versatile, biocompatible properties, superparamagnetic iron oxide (SPIO) or ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are utilized for detecting and tracing cells or tumors in vivo. Here, we developed an innoxious and concise synthesis approach for a novel B-cell lymphoma (Bcl)-2 monoclonal antibody-functionalized USPIO nanoparticle coated with an amphiphilic polymer (carboxylated polyethylene glycol monooleyl ether [OE-PEG-COOH]). These nanoparticles can be effectively internalized by beta cells and label primary islet cells, at relatively low iron concentration. The biocompatibility and cytotoxicity of these products were investigated by comparison with the commercial USPIO product, FeraSpin™ S. We also assessed the safe dosage range of the product. Although some cases showed a hypointensity change at the site of transplant, a strong magnetic resonance imaging (MRI) was detectable by a clinical MRI scanner, at field strength of 3.0 Tesla, in vivo, and the iron deposition/attached in islets was confirmed by Prussian blue and immunohistochemistry staining. It is noteworthy that based on our synthesis approach, in future, we could exchange the Bcl-2 with other probes that would be more specific for the targeted cells and that would have better labeling specificity in vivo. The combined results point to the promising potential of the novel Bcl-2-functionalized PEG-USPIO as a molecular imaging agent for in vivo monitoring of islet cells or other cells.