The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
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DOI：
10.1016/j.toxicon.2009.01.014
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发表时间：
2009-04-01
期刊：
影响因子：
2.8
通讯作者：
Blaha, Ludek
中科院分区：
文献类型：
--
作者：
Blahova, Lucie;Oravec, Michal;Blaha, Ludek
Cylindrospermopsin (CYN) 是一种新出现的致癌生物碱，最初在热带蓝藻 Cylindrospermopsis raciborskii 中发现。最近的一些研究报告称，欧洲国家的其他蓝藻也产生这种毒素。在此，我们报告了免疫化学 ELISA 测定与液相色谱/质谱 (LC/MS) 方法对捷克共和国和中欧样品中 CYN 分析的比较。我们分析了从 32 个地区采集的 56 个原湖水样本。 CYN 在 3 个地点与束丝藻 (Aphanizomenon sp.) 一起被发现。次主导水华。 ELISA 和 LC/MS 的结果显示出良好的定性一致性。然而，通过 ELISA 测定的浓度 (0.4-4 μg/L) 系统地高于通过 LC/MS 测定的浓度 (0.01-0.3 μg/L)。方法之间的差异可能归因于基质效应（ELISA 和 LC/MS 中）或 ELISA 与其他未鉴定的 CYN 衍生物的交叉反应性。我们的研究首次证明 CYN 在中欧出现。鉴于其疑似致癌性，需要进一步研究这种蓝藻毒素的分布、浓度和风险。 (C) 2009 Elsevier Ltd. 保留所有权利。
Cylindrospermopsin (CYN) is a newly emerging carcinogenic alkaloid originally identified in tropical cyanobacteria Cylindrospermopsis raciborskii. Some recent studies reported production of this toxin also by other cyanobacteria in European countries. Here we report comparison of the immunochemical ELISA determination with the liquid chromatography/mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. We have analysed 56 samples of raw lake waters collected from 32 localities. CYN was found at 3 localities with Aphanizomenon sp. sub-dominated water blooms. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA (0.4-4 mu g/L) were systematically higher than concentrations determined by LC/MS (0.01-0.3 mu g/L). Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe. With respect to its suspected carcinogenicity, further research on distribution, concentrations and risks of this cyanobacterial toxin is needed. (C) 2009 Elsevier Ltd. All rights reserved.