A mosquito entomoglyceroporin, Aedes aegypti AQP5, participates in water transport across the Malpighian tubules of larvae
A mosquito entomoglyceroporin, Aedes aegypti AQP5, participates in water transport across the Malpighian tubules of larvae
复制标题
DOI：
10.1242/jeb.158352
复制
发表时间：
2017-10-01
影响因子：
2.8
通讯作者：
Donini, Andrew
中科院分区：
文献类型：
--
作者：
Misyura, Lidiya;Yerushalmi, Gil Y.;Donini, Andrew
埃及伊蚊是传播寨卡热、登革热、基孔肯雅热和黄热病等虫媒病毒疾病的主要媒介。幼虫生活在低渗透的淡水栖息地，在那里它们的体液会因渗透水的流入而被稀释。马氏管通过清除血淋巴中多余的水分，帮助维持离子和渗透平衡；然而，这种运动的跨细胞途径仍未解决。水通道蛋白是一种跨膜通道，被认为允许水从血淋巴跨细胞运输到马氏管管腔。埃及斑潜蝇水通道蛋白5(AaAQP5)的免疫定位显示，幼虫的马氏管主细胞表达AaAQP5，并定位于顶膜和基侧膜。用双链RNA敲除AaAQP5可降低幼虫存活率，减少马氏管分泌液体、K+和Na+的速率，并降低血淋巴中的氯离子浓度。这些发现表明，AaAQP5参与了埃及斑潜蝇幼虫马氏管的跨细胞水分运输，其中AaAQP5的整体表达对幼虫的生存至关重要。
The mosquito Aedes aegypti is the primary vector for arboviral diseases such as Zika fever, dengue fever, chikungunya and yellow fever. The larvae reside in hypo-osmotic freshwater habitats, where they face dilution of their body fluids from osmotic influx of water. The Malpighian tubules help maintain ionic and osmotic homeostasis by removing excess water from the hemolymph; however, the transcellular pathway for this movement remains unresolved. Aquaporins are transmembrane channels thought to permit transcellular transport of water from the hemolymph into the Malpighian tubule lumen. Immunolocalization of A. aegypti aquaporin 5 (AaAQP5) revealed expression by Malpighian tubule principal cells of the larvae, with localization to both the apical and basolateral membranes. Knockdown of AaAQP5 with doubl-estranded RNA decreased larval survival, reduced rates of fluid, K+ and Na+ secretion by the Malpighian tubules, and reduced Cl- concentrations in the hemolymph. These findings indicate that AaAQP5 participates in transcellular water transport across the Malpighian tubules of larval A. aegypti where global AaAQP5 expression is important for larval survival.