Possible involvement of Fas-mediated apoptosis in eye muscle tissue from patients with thyroid-associated ophthalmopathy
Possible involvement of Fas-mediated apoptosis in eye muscle tissue from patients with thyroid-associated ophthalmopathy
复制标题
DOI：
10.1089/thy.1998.8.311
复制
发表时间：
1998-04-01
期刊：
影响因子：
6.6
通讯作者：
Nonaka, K
中科院分区：
文献类型：
--
作者：
Koga, M;Hiromatsu, Y;Nonaka, K
为了探讨甲状腺相关眼病（TAO）的发病机制中可能涉及的细胞凋亡，我们研究了Fas，Fas配体（FasL），Bcl-2的表达与TAO患者的眼外肌组织的免疫组化使用单克隆抗体抗Fas，FasL，Bcl-2。DNA原位末端标记法检测细胞凋亡。19例TAO患者中有17例眼外肌组织中检测到细胞凋亡，7例正常对照组中有4例检测到细胞凋亡。TAO眼肌纤维和TAO间质细胞中凋亡细胞核的平均百分比分别为12.1%和16.4%，而正常眼肌纤维和间质细胞中分别为2.2%和0.4%。眼肌纤维中凋亡细胞核的百分率与眼肌组织增大程度相关（r = 0.47，p < 0.05）。18例TAO患者中有11例眼外肌纤维表面有Fas表达，而正常眼外肌组织未见Fas表达。TAO和正常眼外肌组织均不表达Bcl-2。在TAO患者眼外肌组织中的浸润单核细胞中检测到Fast。这些数据表明，Fas介导的凋亡可能发生在TAO患者的眼外肌组织中，并可能参与了TAO的晚期。
In order to investigate the possible involvement of apoptosis in the pathogenesis of thyroid-associated ophthalmopathy (TAO), we studied the expression of Fas, Fas ligand (FasL), and Bcl-2 in extraocular muscle tissues from patients with TAO by immunohistochemistry using monoclonal antibodies against Pas, FasL, and Bcl-2. Apoptosis was detected by in situ end-labeling of fragmented DNA. Apoptosis was detected in extraocular muscle tissues from 17 of 19 patients with TAO and, to a smaller degree in 4 of 7 normal control subjects. The mean percentages of apoptotic nuclei were 12.1% in TAO eye muscle fibers and 16.4% in TAO interstitial cells, compared with 2.2% and 0.4% in normal eye muscle fibers and interstitial cells, respectively. The percentage of apoptotic nuclei in eye muscle fibers correlated with the enlargement of eye muscle tissue (r = 0.47, p < 0.05). Fas was demonstrated on the surfaces of extraocular muscle fibers in 11 of 18 patients with TAO, but not in normal extraocular muscle tissues. Neither TAO nor normal extraocular muscle tissues expressed Bcl-2. Fast was detected in infiltrating mononuclear cells in extraocular muscle tissue from a patient with TAO. These data suggest that Fas-mediated apoptosis may occur in extraocular muscle tissue from patients with TAO and may be involved in the late stage of TAO.