Flow Cytometry Can Diagnose Classical Hodgkin Lymphoma in Lymph Nodes With High Sensitivity and Specificity
Flow Cytometry Can Diagnose Classical Hodgkin Lymphoma in Lymph Nodes With High Sensitivity and Specificity
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DOI：
10.1309/ajcpw3un9dyldspb
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发表时间：
2009-03-01
影响因子：
3.5
通讯作者：
Wood, Brent L.
中科院分区：
文献类型：
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作者：
Fromm, Jonathan R.;Thomas, Anju;Wood, Brent L.
经典霍奇金淋巴瘤（CHL）的诊断是在组织切片中进行的，因为试图通过流式细胞术（FC）鉴定淋巴结中的肿瘤性霍奇金和里德斯滕贝格（HRS）细胞一直不成功。然而，我们最近证明，HRS细胞可以通过FC识别，通常以T细胞-HRS细胞的形式存在。在这项研究中，我们检查了一种新的9色（CD 95-太平洋蓝/CD 64-异硫氰酸荧光素/CD 30-藻红蛋白[PE]/CD 45-PE-德克萨斯红/CD 40-PE花青[Cy]5.5/CD 20-PECy 7/CD 15-别藻蓝蛋白[APC]/CD 71-APC-AlexaFluor A700/CD 5-APC-Cv 7）单管FC测定法诊断淋巴结中CHL的有用性。我们使用FC检测来确定279个盲法鉴定的和141个选择的（用于标本炒作或细胞修复形态学特征提示CHL）组织的诊断灵敏度和特异性。在53例经形态学确诊的CHL病例中（10例在CHL组; 43例在选定组），FC检测诊断的敏感性和特异性分别为88.7%和100%。随着目前8色（或更多）临床流式细胞仪的可用性，该检测现在可以应用于常规免疫表型和确认CHL的诊断或作为免疫组化分析的辅助手段。
The diagnosis of classical Hodgkin lymphoma (CHL) has been made in tissue sections as attempts to identify neoplastic Hodgkin and Reed Sternberg (HRS) cells in lymph nodes by flow cytometry (FC) have been unsuccessful. However, we have recently demonstrated that HRS cells can be identified by FC, often present as T-cell-HRS-cell rosettes. In this study, we examined the usefulness of a novel 9-color (CD95-Pacific blue/CD64-fluorescein isothiocyanate/CD30-phycoelythrin [PE]/CD45-PE-Texas red/CD40-PE cyanine [Cy]5.5/CD20-PECy7/CD15-allophycocyanin [APC]/CD71-APC-AlexaFluor A700/CD5-APC-Cv7), single tube FC assay to diagnose CHL in lymph nodes. We used the FC assay to determine diagnostic sensitivity and specificity in 279 blindly identified and 141 selected (for specimen hype or cytopreparation morphologic features suggesting CHL) tissues. Of the 53 morphologically defined CHL cases identified (10 in the unselected group; 43 in the selected group), the FC assay diagnostic sensitivity and specificity were 88.7% and 100%, respectively. With the current availability of 8 (or more) color clinical flow cytometers, this assay can now be applied to routinely immunophenotype and confirm a diagnosis of CHL or as an adjunct to immunohistochemical analysis.