Clostridium botulinum C2 toxin -: New insights into the cellular up-take of the actin-ADP-ribosylating toxin
Clostridium botulinum C2 toxin -: New insights into the cellular up-take of the actin-ADP-ribosylating toxin
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DOI：
10.1078/1438-4221-00305
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发表时间：
2004-04-01
影响因子：
4.1
通讯作者：
Barth, H
中科院分区：
文献类型：
--
作者：
Aktories, K;Barth, H
肉毒梭菌C2毒素是二元肌动蛋白-ADP-核糖基化毒素家族的成员。它由酶组分C2 I和分离的结合，ng/易位组分C2 II组成。经蛋白水解活化后，C2 II形成七聚体并与碳水化合物细胞表面受体结合。在C2 I附着后，毒素复合物被内吞以到达早期内体。在低pH值的内体中，C2 II-七聚体插入膜中，形成孔并将C2 I递送到胞质溶胶中。在这里，C2 I ADP-核糖基化肌动蛋白在Arg 177阻断肌动蛋白聚合和诱导肌动蛋白丝解聚。简要综述了C2毒素的主要特性，并讨论了分子伴侣参与毒素摄取过程的新发现。
Clostridium botulinum C2 toxin is a member of the family of binary actin-ADP-ribosylating toxins. It consists of the enzyme component C2I, and the separated bind,ng/translocation component C2II. Proteolytically activated,C2II forms heptamers and binds to a carbohydrate cell surface receptor. After attachment of C2I, the toxin complex is endocytosed to reach early endosomes. At low pH of endosomes, C2II-heptamers insert into the membrane, form pores and deliver C2I into the cytosol. Here, C2I ADP-ribosylates actin at Arg177 to block actin polymerization and to induce depolymerization of actin filaments. The mini-review describes main properties of C2 toxin and discusses new findings on the involvement of chaperones in the up-take process of the toxin.