Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis
Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis
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DOI：
10.1038/s42003-019-0646-5
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发表时间：
2019-11-04
影响因子：
5.9
通讯作者：
Sugimoto, Keiko
中科院分区：
文献类型：
--
作者：
Rymen, Bart;Kawamura, Ayako;Sugimoto, Keiko
植物体细胞在严重受损时，会重新编程并再生新的组织或器官。这些生理过程与动态转录反应有关，但基于染色质的调控如何促进伤口诱导的基因表达变化和随后的细胞重编程仍然未知。在本研究中，我们研究了组蛋白修饰H3K9/14ac、H3K27ac、H3K4me3、H3K27me3和H3K36me3的时间动态，并分析了它们在损伤后早期时间点与基因表达的相关性。我们发现，在损伤快速诱导的数千个基因中，大多数在损伤之前和/或之后不久被标记为H3K9/14ac和H3K27ac，其中包括关键的伤口诱导重编程基因，如WIND1、ERF113/RAP2.6 L和LBD16。我们的数据进一步表明，抑制gnat - myst介导的组蛋白乙酰化强烈地阻断了伤口诱导的转录激活以及伤口部位的愈伤组织形成。因此，这项研究揭示了植物损伤诱导细胞重编程的关键表观遗传机制。
Plant somatic cells reprogram and regenerate new tissues or organs when they are severely damaged. These physiological processes are associated with dynamic transcriptional responses but how chromatin-based regulation contributes to wound-induced gene expression changes and subsequent cellular reprogramming remains unknown. In this study we investigate the temporal dynamics of the histone modifications H3K9/14ac, H3K27ac, H3K4me3, H3K27me3, and H3K36me3, and analyze their correlation with gene expression at early time points after wounding. We show that a majority of the few thousand genes rapidly induced by wounding are marked with H3K9/14ac and H3K27ac before and/or shortly after wounding, and these include key wound-inducible reprogramming genes such as WIND1, ERF113/RAP2.6 L and LBD16. Our data further demonstrate that inhibition of GNAT-MYST-mediated histone acetylation strongly blocks wound-induced transcriptional activation as well as callus formation at wound sites. This study thus uncovered a key epigenetic mechanism that underlies wound-induced cellular reprogramming in plants.