Extracorporeal shock waves: From lithotripsy to anti-inflammatory action by NO production
Extracorporeal shock waves: From lithotripsy to anti-inflammatory action by NO production
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DOI：
10.1016/j.niox.2004.12.005
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发表时间：
2005-03-01
影响因子：
3.9
通讯作者：
Suzuki, H
中科院分区：
文献类型：
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作者：
Mariotto, S;Cavalieri, F;Suzuki, H
在低能量密度（0.03 mJ/mm（2））下，最初为临床碎石术开发的体外冲击波（ESW）已成功用于软组织的抗炎治疗。由于一氧化氮在炎症中起着关键作用，我们假设ESW可以增加细胞中NO的产生。以人脐静脉内皮细胞为模型系统，我们观察到低能量密度的ESW可迅速诱导eNOS活性增强。在这些细胞中，eNOS活性受到酪氨酸和丝氨酸磷酸化的调节。ESW使eNOS转变为较少酪氨酸磷酸化的形式，而不影响其丝氨酸磷酸化，从而解释其快速酶激活。LPS/IFN-γ处理的人脐静脉内皮细胞诱导eNOS活性的快速抑制和伴随的NF-B-K活化，这被ESW处理有效地抵消。因此，本研究结果表明，临床观察到的ESW抗炎作用的分子机制应包括eNOS的酪氨酸去磷酸化、NO产生的连续增加和NF-B-K活化的抑制。(c)2004年爱思唯尔公司All rights reserved.
At low energy density (0.03 mJ/mm(2)), extracorporeal shock waves (ESW), originally developed for clinical lithotripsy, have successfully been used for anti-inflammatory treatment of soft tissues. Since nitric oxide plays a critical role in inflammation, we hypothesized for ESW to increase NO production in cells. Using human umbilical vein enclothelial cells as a model system, we observed that ESW, at low energy density, rapidly induced an enhancement of eNOS activity. In these cells, eNOS activity is inodulated by tyrosine- and serine-phosphorylation. ESW shifted eNOS to a less-tyrosine-phosphorylated form, Without affecting its serine-phosphorylation, thus accounting for its rapid enzyme activation. LPS/IFN-gamma treatment of human umbilical vein enclothelial cells induced a rapid inhibition of eNOS activity and concomitant NF-B-K activation which were efficiently counteracted by ESW treatment. Therefore, the present results indicate that the molecular mechanism of clinically observed anti -inflammatory action of ESW should include tyrosine-dephosphorylation of eNOS, a successive increase in NO production and suppression of NF-B-K activation. (c) 2004 Elsevier Inc. All rights reserved.