Experimental transmission in guinea pigs of H9N2 avian influenza viruses from indoor air of chicken houses
Experimental transmission in guinea pigs of H9N2 avian influenza viruses from indoor air of chicken houses
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DOI：
10.1016/j.virusres.2012.09.003
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发表时间：
2012-12-01
期刊：
影响因子：
5
通讯作者：
Chai, Tongjie
中科院分区：
文献类型：
--
作者：
Lv, Jing;Wei, Baozhi;Chai, Tongjie
本研究的目的是确定H9 N2禽流感病毒（AIV）的空气来源的传播特性。2009年至2010年期间，从鸡舍中分离出8株H9 N2禽流感病毒。我们分析了这些菌株的致病性和致病性。此外，还在实验条件下测定了两种空气传播分离株在豚鼠中的传播特征。系统进化分析表明，8个分离物HA和NA基因的同源性分别为95.4-99.7%和86.6-99.8%。它们能够在豚鼠肺组织中复制而无需预先适应。两株气传分离株均能通过直接接触在豚鼠间传播。在气溶胶接触动物中未检测到感染，而在隔离器的空气中检测到H9 N2 AIV气溶胶。气溶胶感染剂量实验表明，H9 N2亚型禽流感病毒气溶胶对豚鼠的半数感染剂量（ID 50）为3.58 × 10（6）拷贝，说明在实验条件下，一定浓度的气溶胶可感染豚鼠。总之，H9 N2 AIV气溶胶对哺乳动物具有感染性，这表明需要紧急关注其传播。(c)2012爱思唯尔有限公司版权所有。
This study aimed to determine the transmission characteristics of H9N2 avian influenza viruses (AIVs) derived from the air. Eight H9N2 AIVs were isolated from chicken houses between 2009 and 2010. We analyzed the phylogenic and pathogenic traits of these isolates. What is more, transmission characteristics in guinea pigs of two airborne isolates were determined in experimental conditions. Phylogenetic analyses indicated that the homologies of HA and NA genes of eight isolates were 95.4-99.7% and 86.6-99.8% respectively. They were able to duplicate in lung tissues of guinea pigs without prior adaptation. Two airborne isolates could both transmit among guinea pigs by direct contact. No infection was detected in aerosol contact animals while H9N2 AIV aerosols were detected in the air of isolators. Aerosol infection dose experiment showed that aerosol median infective dose (ID50) of H9N2 AIV to guinea pigs was 3.58 x 10(6) copies, demonstrating that the aerosols could infect guinea pigs at certain concentrations in experimental condition. In conclusion, H9N2 AIV aerosols were infectious to mammals, suggesting that urgent attention will need to be paid to its transmission. (c) 2012 Elsevier B.V. All rights reserved.