Fluorescence Discrimination of Cancer from Inflammation by Molecular Response to COX-2 Enzymes
Fluorescence Discrimination of Cancer from Inflammation by Molecular Response to COX-2 Enzymes
复制标题
通过对 COX-2 酶的分子反应来荧光区分癌症和炎症
DOI：
10.1021/ja4085308
复制
发表时间：
2013-11-20
影响因子：
15
通讯作者：
Peng, Xiaojun
中科院分区：
文献类型：
--
作者：
Zhang, Hua;Fan, Jiangli;Peng, Xiaojun
以快速、灵敏和定量的方式从炎症和正常组织中准确识别癌症对于手术期间的癌症诊断和切除是重要的。在这里，我们报告使用环氧合酶-2作为一个标志物，用于识别癌症的炎症和一种新的智能考克斯-2特异性荧光探针（NANQ-IMC 6）的设计。在考克斯-2过表达的炎症和癌症中，探针的荧光都被“打开”。有趣的是，考克斯-2表达水平不同的这两个位点的荧光发射是完全不同的。因此，NANQ-IMC 6不仅可以区分正常细胞/组织与癌细胞/组织，而且还可以通过NANQ-IMC 6对考克斯-2酶的不同荧光识别来区分后者与炎症病变部位。在用NANQ-IMC 6溶液喷雾后，癌组织、发炎组织和正常组织可以使用在365 nm处发射的手持式紫外灯通过肉眼在体内准确地区分。因此，该探针可能具有从癌症炎症诊断到在手术中指导肿瘤切除的潜在应用。
Accurate identification of cancer from inflammation and normal tissue in a rapid, sensitive, and quantitative fashion is important for cancer diagnosis and resection during surgery. Here we report the use of cyclooxygenase-2 as a marker for identification of cancer from inflammation and the design of a novel smart COX-2-specific fluorogenic probe (NANQ-IMC6). The probe's fluorescence is "turned on" in both inflammations and cancers where COX-2 is overexpressed. Intriguingly, the fluorescent emission is quite different at these two sites with different expression level of COX-2. Hence, NANQ-IMC6 can not only distinguish normal cells/tissues from cancer cells/tissues but also distinguish the latter from sites of inflammation lesions by the different fluorescence recognition of NANQ-IMC6 for COX-2 enzymes. Following spraying with the NANQ-IMC6 solution, cancerous tissue, inflamed tissues, and normal tissues can be accurately discriminated in vivo by the unaided eye using a hand-held ultraviolet lamp emitting at 365 nm. So the probe may have potential application varying from cancer inflammation diagnosis to guiding tumor resection during surgery.