Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco
Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco
复制标题
DOI：
10.1016/j.jplph.2010.07.014
复制
发表时间：
2011-03-01
影响因子：
4.3
通讯作者：
Graciela Orellano, Elena
中科院分区：
文献类型：
--
作者：
Damian Daurelio, Lucas;Petrocelli, Silvana;Graciela Orellano, Elena
植物持续暴露于病原体的挑战。病原微生物最常见的防御反应是非宿主反应，通常伴随着转录变化。为了鉴定与非宿主抗性相关的基因，我们评估了用Xanthomonas axonopodis pv.感染后的烟草转录组谱。citri（Xac）是一种非寄主植物病原细菌。利用cDNA扩增片段长度多态性（cDNA amplified fragment length polymorphism）技术，分析了Xac侵染烟草后2、8和24 h的差异表达。从筛选的总共2087个转录衍生片段（TDFs）（约占烟草转录组的20%）中，316个TDFs显示出差异表达。基于序列相似性，82个差异TDFs被鉴定并分配到不同的功能类别：56个与已知功能的基因同源，12个与未知功能的蛋白质同源，14个没有匹配。用选择的转录物进行实时PCR以确认获得的表达模式。这些结果揭示了烟草中与植物-病原菌互作中的非寄主抗性相关的新基因。这些新基因可用于未来的非寄主抗病分子育种策略。(C)2010年Elsevier GmbH。All rights reserved.
Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24 h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance. (C) 2010 Elsevier GmbH. All rights reserved.