Exercise Increases Glucose Transporter-4 Levels on Peripheral Blood Mononuclear Cells.
Exercise Increases Glucose Transporter-4 Levels on Peripheral Blood Mononuclear Cells.
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DOI：
10.1249/mss.0000000000001528
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发表时间：
2018-05
影响因子：
4.1
通讯作者：
Dunlap KL
中科院分区：
文献类型：
--
作者：
Sticka KD;Schnurr TM;Jerome SP;Dajles A;Reynolds AJ;Duffy LK;Knall CM;Dunlap KL
葡萄糖转运蛋白4 （GLUT4）在2型糖尿病的病理生理中起关键作用。GLUT4在运动时上调，增强骨骼肌组织的细胞葡萄糖运输。这种机制在胰岛素抵抗个体中似乎保持不变。由于肌肉活检的侵入性，其机制的细节尚不清楚，并且具有挑战性。外周血单核细胞（PBMC）具有胰岛素敏感的GLUT4活性，可作为研究骨骼肌GLUT4的代理组织。本研究的目的是探讨PBMC中的GLUT4是否受到条件作用的影响。我们从阿拉斯加费尔班克斯大学招募了16名越野跑和滑雪队的学生运动员，以及15名年龄和性别匹配的久坐不动的学生。采用单核细胞分离管采集PMBC。使用市售的酶联免疫吸附剂测定GLUT4浓度。此外，我们还检测了PBMC GLUT4与胰岛素抵抗常见指标之间的相关性。结果表明，条件运动员的PBMC GLUT4水平明显高于久坐不动的运动员，这与在肌细胞中记录的结果相似。观察到女性的PBMC GLUT4水平高于男性。PBMC GLUT4与糖化血红蛋白（HbA1c）、葡萄糖、胰岛素、HOMA-IR、BMI或体脂之间未发现相关性。本研究为探索PBMC作为研究GLUT4对运动或其他非胰岛素因素反应的代理组织提供了证据。
Glucose transporter 4 (GLUT4) plays a key role in the pathophysiology of type 2 diabetes. GLUT4 is upregulated in response to exercise, enhancing cellular glucose transport in skeletal muscle tissue. This mechanism appears to remain intact in individuals with insulin resistance. Details of the mechanism are poorly understood and are challenging to study due to the invasive nature of muscle biopsy. Peripheral blood mononuclear cells (PBMC) have documented insulin-sensitive GLUT4 activity and may serve as a proxy tissue for studying skeletal muscle GLUT4. The purpose of this study was to investigate whether GLUT4 in PBMC is affected by conditioning. We recruited sixteen student athletes from the cross-country running and skiing teams and fifteen sedentary students matched for age and sex from the University of Alaska Fairbanks. PMBC were collected with mononuclear cell separation tubes. GLUT4 concentrations were measured using a commercially available enzyme linked immunosorbant assay. Additionally, correlations between PBMC GLUT4 and common indicators of insulin resistance were examined. Results indicate significantly higher PBMC GLUT4 levels in conditioned athletes than in their sedentary counterparts, similar to what has been documented in myocytes. Females were observed to have higher PBMC GLUT4 levels than males. Correlations were not detected between PBMC GLUT4 and hemoglobin A1c (HbA1c), glucose, insulin, HOMA-IR, BMI, or body fat. This study provides evidence to support exploration of PBMC as a proxy tissue for studying GLUT4 response to exercise or other non-insulin factors.