Cell patterning in pigment-chimeric eyes in Xenopus: germinal transplants and their contributions to growth of the pigmented retinal epithelium.
Cell patterning in pigment-chimeric eyes in Xenopus: germinal transplants and their contributions to growth of the pigmented retinal epithelium.
复制标题
非洲爪蟾色素嵌合眼的细胞模式：生发移植及其对色素视网膜上皮生长的贡献。
DOI：
10.1073/pnas.84.10.3302
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发表时间：
1987
影响因子：
11.1
通讯作者：
Mason,BJ
中科院分区：
文献类型：
--
作者：
Hunt,RK;Cohen,JS;Mason,BJ
我们研究了从30-38期供体胚胎原位移植到28-38期白化宿主的一小群色素生发细胞为非洲爪蟾生长的幼虫眼睛的色素视网膜上皮提供新的有丝分裂后细胞的过程。在绝大多数嵌合眼中，移植体愈合后在寄主生发带的预定背侧或前部位置占据一个小弧形区域。在随后几周的过程中，移植的生发细胞在色素视网膜上皮的远端边缘添加了新的有丝分裂静止细胞，因此在生长中的幼虫眼睛上产生了一个细长的黑色部分。移植物区边界的细胞细节随着时间的推移是稳定的；这些标记的积累提供了移植生长历史的总结性记录——在老眼的近远轴上。两组移植病例之间的差异表明，生发细胞生长的细节存在一定程度的不确定性。
We have examined the process by which small groups of pigmented germinal cells transplanted orthotopically from stage 30-38 donor embryos into stage 28-38 albino hosts contribute new postmitotic cells to the pigmented retinal epithelium of the growing larval eye in Xenopus. In the great majority of chimeric eyes, the transplant healed to occupy a small arc-territory at the intended dorsal or anterior position on the host germinal zone. Over the course of subsequent weeks, the transplanted germinal cells added new mitotically quiescent cells to the distal rim of the pigmented retinal epithelium and so gave rise to an elongating black sector on the growing larval eye. Cellular details at the boundaries of the graft-derived sector were stable over time; the accumulation of such landmarks provided a summary record--in the proximodistal axis of the older eye--of the growth history of the transplant. Case-to-case variation among both groups of transplants suggested a measure of indeterminancy in the details of germinal cell growth.