Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis
Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis
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DOI：
10.1063/1.4732800
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发表时间：
2012-09-01
期刊：
影响因子：
3.2
通讯作者：
Xuan, Xiangchun
中科院分区：
文献类型：
--
作者：
Patel, Saurin;Showers, Daniel;Xuan, Xiangchun
活细胞和死亡细胞的分离对于早期疾病的诊断和药物筛选等有效性测试是至关重要的。本工作展示了一种新的微流控方法，通过活性来进行酵母细胞的介电泳法分离。它利用水库-微通道交界处固有的电场梯度诱导的细胞介电，选择性地捕获死亡的酵母细胞，并在水库内将它们与活的酵母细胞连续分离。因此，这种方法被称为基于储备库的介电泳法(RDEP)。与现有的介电泳法相比，它具有独特的优势，如占用零通道空间和消除微通道内的任何机械或电气部件。这种rDEP细胞分选器可以很容易地与其他组件集成到芯片实验室设备中，用于生物医学诊断和治疗。(C)2012年美国物理研究所。[http://dx.doi.org/10.1063/1.4732800]
Separating live and dead cells is critical to the diagnosis of early stage diseases and to the efficacy test of drug screening, etc. This work demonstrates a novel microfluidic approach to dielectrophoretic separation of yeast cells by viability. It exploits the cell dielectrophoresis that is induced by the inherent electric field gradient at the reservoir-microchannel junction to selectively trap dead yeast cells and continuously separate them from live ones right inside the reservoir. This approach is therefore termed reservoir-based dielectrophoresis (rDEP). It has unique advantages as compared to existing dielectrophoretic approaches such as the occupation of zero channel space and the elimination of any mechanical or electrical parts inside microchannels. Such an rDEP cell sorter can be readily integrated with other components into lab-on-a-chip devices for applications to biomedical diagnostics and therapeutics. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4732800]