Validation of Power Doppler Versus Contrast-Enhanced Magnetic Resonance Imaging Quantification of Joint Inflammation in Murine Inflammatory Arthritis
Validation of Power Doppler Versus Contrast-Enhanced Magnetic Resonance Imaging Quantification of Joint Inflammation in Murine Inflammatory Arthritis
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DOI：
10.1002/jbmr.2392
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发表时间：
2015-04-01
影响因子：
6.2
通讯作者：
Schwarz, Edward M.
中科院分区：
文献类型：
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作者：
Bouta, Echoe M.;Banik, Peony D.;Schwarz, Edward M.
滑膜体积的对比增强磁共振成像(CE-MRI)是量化关节炎症的放射学金标准；然而，成本限制了它的使用。因此，我们研究了肿瘤坏死因子转基因(TNF-TG)小鼠滑膜炎的能量多普勒超声(PD-US)结果是否与CE-MRI相关。对肿瘤坏死因子-TG小鼠进行膝关节PD-US检查，测量关节间隙体积(JSV)和能量多普勒体积(PDV)，并与CE-MRI测定的滑膜体积进行相关分析。CD31免疫组织化学染色证实PD信号。滑膜体积与JSV和PDV显著相关(p<；0.01)。CD31(+)血管见于关节面炎性滑膜内，与PD信号区相对应。JSV和PDV是关节炎症的有效指标，与CE-MRI所确定的滑膜体积相关，并与血管相关。鉴于PD-US的出现是关节炎症的非定量结果，我们发现JSV和PDV对于动物模型的纵向研究是可行的和高成本效益的。此外，鉴于PD-US在标准临床实践中的使用越来越多，JSV和PDV可以转化为更好地量化类风湿性关节炎(RA)患者的关节炎症和治疗反应。(C)2014年美国骨与矿物研究学会。
Contrast-enhancement magnetic resonance imaging (CE-MRI) of synovial volume is the radiographic gold standard to quantify joint inflammation; however, cost limits its use. Therefore, we examined if power Doppler-ultrasound (PD-US) outcomes of synovitis in tumor necrosis factor transgenic (TNF-Tg) mice correlate with CE-MRI. TNF-Tg mice underwent PD-US of their knees to measure the joint space volume (JSV) and power Doppler volume (PDV), and the results were correlated with synovial volume determined by CE-MRI. Immunohistochemistry for CD31 was performed to corroborate the PD signal. Synovial volume strongly correlated with both JSV and PDV (p < 0.01). CD31(+) blood vessels were observed in inflamed synovium proximal to the joint surface, which corresponded to areas of intense PD signals. JSV and PDV are valid measures of joint inflammation that correlate with synovial volume determined by CE-MRI and are associated with vascularity. Given the emergence of PD-US as a nonquantitative outcome of joint inflammation, we find JSV and PDV to be feasible and highly cost-effective for longitudinal studies in animal models. Furthermore, given the increasing use of PD-US in standard clinical practice, JSV and PDV could be translated to better quantify joint flare and response to therapy in patients with rheumatoid arthritis (RA). (c) 2014 American Society for Bone and Mineral Research.