The Panicle Blast Resistance Mechanism of qPbm11 in the Rice Cultivar Miyazaki-mochi is Independent from that of Pb1
The Panicle Blast Resistance Mechanism of qPbm11 in the Rice Cultivar Miyazaki-mochi is Independent from that of Pb1
复制标题
水稻品种 Miyazaki-mochi 中 qPbm11 的抗穗瘟病机制独立于 Pb1
DOI：
10.6090/jarq.53.289
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
N. Hayashi
中科院分区：
文献类型：
--
作者：
H. Inoue;N. Hayashi
了解稻瘟病的抗性机制对于对抗稻瘟病是至关重要的，稻瘟病对水稻产量造成了最严重的影响，对于利用稻瘟病抗性(R)基因进化出成功的稻瘟病抗性育种方案至关重要。抗稻瘟病品种Miyazakimochi的qPbm11位点具有与众所周知的定量抗穗基因Pb1相似的穗抗性。qPbm11和Pb1在水稻穗瘟病抗性、数量抗性和基因组上的接近性上表现出相似性。Pb1抗性依赖于转录因子WRKY45的表达。为了确定qPbm11位点的抗性机制，我们通过农杆菌介导的转化，下调了宫崎麻麻的WRKY45位点，并对其穗部抗性进行了调查。转WRKY45 RNAi基因的宫崎麻子植株表现出与WT相似的穗抗性水平，表明qPbm11位点不依赖于Pb1抗性途径。结果表明，利用金字塔技术克隆qpbm11介导的抗性基因和Pb1介导的抗性基因可以增强水稻穗部抗性。附加关键词：水稻，稻瘟病菌，穗瘟病菌，QTL，水杨酸，抗性基因
Understanding the mechanism of disease resistance is critical for combating rice blast disease that causes the most deleterious effects on rice yield, and for evolving successful blast tolerance breeding programs using blast resistance (R) genes. The qPbm11 locus in the blast resistant cultivar Miyazakimochi exerts panicle resistance similar to the well-known quantitative panicle resistance gene Pb1. qPbm11 and Pb1 show similarity in panicle blast resistance, quantitative resistance, and proximity of loci on the rice genome. Pb1 resistance is dependent on the expression of transcription factor WRKY45. To identify the resistance mechanism of the qPbm11 locus, we downregulated WRKY45 in the cultivar Miyazaki-mochi through an Agrobacterium-mediated transformation and surveyed its panicle resistance. WRKY45 RNAi transgenic Miyazaki-mochi plants exhibited similar levels of panicle resistance compared to WT, indicating that the qPbm11 locus is independent of the Pb1 resistance pathway. Our results suggested that cloning the genes responsible for qPbm11-mediated resistance along with Pb1 using pyramiding technology could enhance panicle resistance in rice. Discipline: Agricultural Environment Additional key words: Oryza sativa, Pyricularia oryzae, panicle blast, QTL, salicylic acid, resistance gene
影响因子：
11.6
作者：
Shimono, Masaki;Sugano, Shoji;Takatsuji, Hiroshi
通讯作者：
Takatsuji, Hiroshi