MECHANISMS OF RESISTANCE TO ORGANOPHOSPHORUS INSECTICIDES IN CULEX-TARSALIS
MECHANISMS OF RESISTANCE TO ORGANOPHOSPHORUS INSECTICIDES IN CULEX-TARSALIS
复制标题
DOI：
10.1093/jee/68.2.153
复制
发表时间：
1975-01-01
影响因子：
2.2
通讯作者：
GEORGHIOU, GP
中科院分区：
文献类型：
--
作者：
APPERSON, CS;GEORGHIOU, GP
一个多重抗性品系的跗库蚊Coquillett的幼虫中的55.6倍的抗性水平，是由于较慢的渗透和增加的代谢毒物。暴露4 h后，从抗性菌株中回收的水溶性代谢物比敏感菌株多1.9倍，完整的代谢物少0.4倍。从两种菌株中分离出相当数量的对氧磷。在同一时期内，敏感品系的幼虫吸收磷的量是抗性品系的1.3倍.体内试验表明，抗性品系对有机磷化合物的增效作用与DEF（S，S，S-三丁基三硫代磷酸酯）有关，而增效作用与胡椒基丁醚无关.体外试验表明，抗性品系对乙酰胆碱酯酶的抗性不是由于对有机磷抑制不敏感所致.
A 55.6-fold level of resistance to parathion in larvae of a multiresistant strain ofCulex tarsalisCoquillett was shown to be due to slower penetration and increased metabolism of the toxicant. After 4 h exposure, 1.9× more water-soluble metabolites and 0.4× less intact parathion were recovered from the resistant strain relative to the susceptible. Comparable amounts of paraoxon were isolated from both strains. During the same period, larvae of the susceptible strain absorbed 1.3× as much parathion as resistant larvae.In vivotests revealed strong synergism of organophosphorus compounds in the resistant strain by DEF (S, S, S-tributyl phosphorotrithioate) but antagonism or no effect by piperonyl butoxide.In vitroassays indicated that resistance was not due to acetylcholinesterase insensitivity to inhibition by organophosphates.