Cross-protective immunity in calves conferred by a DNA adenine methylase deficient Salmonellaenterica serovar Typhimurium vaccine.
Cross-protective immunity in calves conferred by a DNA adenine methylase deficient Salmonellaenterica serovar Typhimurium vaccine.
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DNA 腺嘌呤甲基化酶缺陷的肠沙门氏菌血清型鼠伤寒疫苗赋予犊牛交叉保护性免疫力。
DOI：
10.1016/j.vaccine.2005.09.022
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发表时间：
2006
期刊：
影响因子：
5.5
通讯作者：
House,JohnK
中科院分区：
文献类型：
--
作者：
Mohler,VirginiaL;Heithoff,DougM;Mahan,MichaelJ;Walker,KeithH;Hornitzky,MichaelA;McConnell,CraigS;Shum,LucyWC;House,JohnK
全球畜牧业集约化生产的趋势与粪便口腔病原体传播增加有关，从而导致沙门氏菌的高流行率。由于许多致病的沙门氏菌血清型往往是畜牧业生产系统的地方病，因此开发一种能够诱导对多个血清型产生免疫的疫苗是可取的。在这里，我们研究了用缺乏DAM腺嘌呤甲基酶(DAM)的改良活沙门氏菌血清鼠伤寒沙门氏菌疫苗株免疫是否对都柏林的异源沙门氏菌具有保护作用。与未接种疫苗的对照动物相比，接种了都柏林毒株的动物表现出显著的临床疾病缓解(态度评分改善，发热和腹泻减少)，并伴随着都柏林粪便脱落和肠系膜淋巴结(MLN)定植的减少。这些数据表明，用DAM−鼠伤寒沙门氏菌疫苗株接种牛，对可归因于都柏林异源挑战的牛的临床疾病具有显著的交叉保护作用。
The global trend towards intensive livestock production is associated with increased fecal oral pathogen transmission resulting in a high prevalence of Salmonella. Since many pathogenic Salmonella serovars are often endemic to livestock production systems, it is desirable to develop a vaccine that is capable of eliciting immunity to more than one serovar. Here we examined whether immunization with a modified live Salmonella enterica serovar Typhimurium vaccine strain lacking the DNA adenine methylase (Dam) conferred protection in calves against a heterologous S. enterica Dublin challenge. Vaccinated animals challenged with a virulent Dublin strain exhibited a significant attenuation of clinical disease (improved attitude scores and reduced fever and diarrhea) and a concomitant reduction in Dublin fecal shedding and colonization of mesenteric lymph nodes (MLN) compared to non-vaccinated control animals. These data suggest that vaccination with a dam−Typhimurium vaccine strain conferred significant cross-protection against clinical disease in cattle attributable to heterologous challenge with Dublin.