Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus
Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus
复制标题
表达猫免疫缺陷病毒Gag前体蛋白的重组猫疱疹病毒1型的构建
DOI：
10.1007/s007050050302
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发表时间：
1998
影响因子：
2.7
通讯作者：
T. Mikami
中科院分区：
文献类型：
--
作者：
E. Sato;N. Yokoyama;K. Maeda;Y. Inoshima;M. Kohmoto;Y. Ikeda;T. Miyazawa;T. Mikami
本研究构建了猫疱疹病毒1型（FHV-1）的缺失突变株和重组FHV-1。缺失突变体是从胸苷激酶（TK）基因的起始密码子到TK基因内的SmaI位点缺失一个区域（367 bp）的病毒，另一个是表达猫免疫缺陷病毒（FIV）Gag蛋白的重组FHV-1，其中编码FIV Gag蛋白的cDNA插入到前者缺失突变体的TK缺失位点。这些病毒分别命名为C7301 ddlTK和C7301 ddlTK-gag。这些病毒在Crandell猫肾细胞中的生长动力学与亲本C7301株相似。通过免疫印迹分析，证实C7301 ddlTK-gag在细胞中表达FIV Gag前体蛋白。
SummaryWe constructed a deletion mutant of feline herpesvirus type 1 (FHV-1) and a recombinant FHV-1. The deletion mutant is the virus with a region (367 bp) deleted from the start codon of thymidine kinase (TK) gene to the SmaI site within the TK gene, and the other is a recombinant FHV-1 expressing Gag protein of feline immunodeficiency virus (FIV), in which a cDNA encoding the Gag protein of FIV was inserted at the TK deletion site of the former deletion mutant. These viruses were designated as C7301ddlTK and C7301ddlTK-gag, respectively. Growth kinetics of these viruses in Crandell feline kidney cells was similar to that of the parent C7301 strain. By immunoblot analysis, C7301ddlTK- gag was confirmed to express the FIV Gag precursor protein in the cells.
细胞相关和无细胞猫免疫缺陷病毒的粘膜传播。
DOI：
10.1089/aid.1997.13.347
发表时间：
1997
期刊：
AIDS research and human retroviruses.
影响因子：
--
作者：
Burkhard,MJ;Obert,LA;O'Neil,LL;Diehl,LJ;Hoover,EA
通讯作者：
Hoover,EA