Confocal Microscopy Used for the Semiautomatic Quantification of the Changes in Aminoacidergic Fibers During Spinal Cord Regeneration
Confocal Microscopy Used for the Semiautomatic Quantification of the Changes in Aminoacidergic Fibers During Spinal Cord Regeneration
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DOI：
10.1007/978-1-4939-0381-8_11
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Celina Rodicio, Maria
中科院分区：
文献类型：
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作者：
Fernandez-Lopez, Blanca;Barreiro-Iglesias, Anton;Celina Rodicio, Maria
脊髓损伤(SCI)会导致哺乳动物的永久性功能缺陷，因此构成了一个公共卫生和经济问题。损伤后观察到不同脊髓神经递质系统的变化。在这一章中，我们描述了一种非常有用的方法来量化脊髓神经递质系统中纤维数量的变化。这种方法结合了共聚焦显微镜和适当的免费计算机软件的使用。将这类研究标准化将非常有助于比较不同研究之间的结果。这种方法也可以用于明视场显微镜，并适用于其他实验条件和动物模型。
Spinal cord injury (SCI) causes permanent functional deficits in mammals, and therefore it constitutes a public health and economic problem. Changes in the different spinal neurotransmitters systems have been observed after the injury. In this chapter, we describe a very useful method to quantify the changes in the number of fibers in the spinal neurotransmitters systems. This method combines confocal microscopy together with the use of appropriate free computer software. Standardizing this type of studies will be very useful to compare results between studies. This method can also be used with bright-field microscopy and be adapted to other experimental conditions and animal models.