SINGLE-CHAIN ANTIGEN-BINDING PROTEINS
SINGLE-CHAIN ANTIGEN-BINDING PROTEINS
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DOI：
10.1126/science.3140379
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发表时间：
1988-10-21
期刊：
影响因子：
56.9
通讯作者：
WHITLOW, M
中科院分区：
文献类型：
--
作者：
BIRD, RE;HARDMAN, KD;WHITLOW, M
单链抗原结合蛋白是一种新型的重组多肽，由抗体可变轻链氨基酸序列（VL）与可变重链序列（VH）通过设计肽段连接而成，VL序列的羧基端与VH序列的氨基端相连。这些蛋白与单克隆抗体具有相同的特异性和亲和力，单克隆抗体的VL和VH序列被用来构建在大肠杆菌中表达的重组基因。设计、构建、合成、纯化和测定了其中的三个蛋白，一个来源于生长激素单克隆抗体的序列，另两个来源于两种不同的荧光素单克隆抗体的序列。预计这些蛋白在许多应用中比单克隆抗体具有显著的优势。
Single-chain antigen-binding proteins are novel recombinant polypeptides, composed of an antibody variable light-chain amino acid sequence (VL) teethered to a variable heavy-chain sequence (VH) by a designed peptide that links the carboxyl terminus of the VL sequence to the amino terminus of the VH sequence. These proteins have the same specificities and affinities for their antigens as the monoclonal antibodies whose VL and VH sequences were used to construct the recombinant genes that were expressed in Escherichia coli. Three of these proteins, one derived for the sequence for a monoclonal antibody to growth hormone and two derived from the sequences of two different monoclonal antibodies to fluorescein, were designed, constructed, synthesized, purified, and assayed. These proteins are expected to have significant advantages over monoclonal antibodies in a number of applications.